Results 31 to 40 of about 7,364,139 (305)

Single-step in vitro reconstitution of the Escherichia coli ribosome mediated by two GTPase factors, EngA and ObgE

eLife
When Escherichia coli ribosomes are assembled in vitro, manipulation of incubation temperature and magnesium ion concentration has been an essential procedure, which is a crucial step for the assembly of active large subunits.
Aya Sato, Weng Yu Lai, Yusuke Sakai, Keiko Masuda, Yoshihiro Shimizu   +4 more
doaj   +1 more source

Synthesis of novel proteins

Current Opinion in Chemical Biology, 1997
De novo and rational protein design are progressing towards the chemical synthesis of proteins with pre-selected structure and function. The data illustrate diverse experimental and computational approaches which test our comprehension of protein structure, hydrophobic core packing and global stability, especially of coiled-coil proteins.
B R, Gibney, F, Rabanal, P L, Dutton
openaire   +2 more sources

Experience With Performing Rheocarna Therapy via the Single‐Needle Method for Treatment of Chronic Limb‐Threatening Ischemia

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction This study investigated the safety and efficacy of single‐needle Rheocarna therapy for chronic limb‐threatening ischemia (CLTI) with wounds. Methods Six patients with CLTI involving ulcers unresponsive to revascularization underwent single‐needle Rheocarna treatment.
Yasutaka Yamauchi, Rie Tanaka, Reona Kawahira, Shingo Nabeta, Kouhei Naoi, Kazuhito Kitajima, Akira Wada, Tomonari Takagi, Norihiko Ohura, Akira Miyamoto   +9 more
wiley   +1 more source

Cell-free protein synthesis of membrane (1,3)-beta-D-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs [PDF]

, 2013
A membrane-embedded curdlan synthase (CrdS) from Agrobacterium is believed to catalyse a repetitive addition of glucosyl residues from UDP-glucose to produce the (1,3)-β-d-glucan (curdlan) polymer.
Mertens, Haydyn D.T., Garajová, Soňa, Hrmova, Maria, Garajova, S., Hrmova, M., Mertens, H., Waters, Shane, Amalraj, Amritha, Nagarajan, Yagnesh, Nagarajan, Y., Waters, S., Periasamy, Agalya, Shadiac, N., Periasamy, A., Shadiac, Nadim, Amalraj, A.   +15 more
core   +1 more source

RETRACTED ARTICLE: Functionalized graphene oxide nanosheets with folic acid and silk fibroin as a novel nanobiocomposite for biomedical applications

Scientific Reports, 2022
In this paper, a novel graphene oxide-folic acid/silk fibroin (GO-FA/SF) nanobiocomposite scaffold was designed and fabricated using affordable and non-toxic materials.
Reza Eivazzadeh-Keihan, Farkhondeh Alimirzaloo, Hooman Aghamirza Moghim Aliabadi, Ehsan Bahojb Noruzi, Ali Reza Akbarzadeh, Ali Maleki, Hamid Madanchi, Mohammad Mahdavi   +7 more
doaj   +1 more source

Effectiveness of Resistance Intradialytic Exercise Compared to Aerobic Intradialytic Exercise for Patients With Chronic Kidney Disease: A Randomized Controlled Clinical Trial

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background Patients with chronic kidney disease undergoing hemodialysis commonly experience reduced physical function, fatigue, poor sleep quality, and impaired health‐related quality of life. Intradialytic exercise has been proposed as a non‐pharmacological strategy to improve these outcomes.
Klebson da Silva Almeida, Gisela Cristiane Miyamoto, Daniel da Costa Torres, Ian Setubal Reis Chaves, Jonas do Carmo Paschoalin, Braúlio Lima Nascimento, Luciana Dias Chiavegato   +6 more
wiley   +1 more source

Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system

eLife
We have developed a deep sequencing-based approach, Rec-Seq, that allows simultaneous monitoring of ribosomal 48S preinitiation complex (PIC) formation on every mRNA in the translatome in an in vitro reconstituted system. Rec-Seq isolates key early steps
Fujun Zhou, Julie M Bocetti, Meizhen Hou, Daoming Qin, Alan G Hinnebusch, Jon R Lorsch   +5 more
doaj   +1 more source

Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae

BMC Biology, 2019
Background Translation of an mRNA in eukaryotes starts at an AUG codon in most cases, but near-cognate codons (NCCs) such as UUG, ACG, and AUU can also be used as start sites at low levels in Saccharomyces cerevisiae.
Shardul D. Kulkarni, Fujun Zhou, Neelam Dabas Sen, Hongen Zhang, Alan G. Hinnebusch, Jon R. Lorsch   +5 more
doaj   +1 more source

Inter-residue through-space scalar ¹⁹F–¹⁹F couplings between CH₂F groups in a protein [PDF]

Magnetic Resonance
Using cell-free protein synthesis, the protein G B1 domain (GB1) was prepared with uniform high-level substitution of leucine by (2S,4S)-5-fluoroleucine (FLeu1), (2S,4R)-5-fluoroleucine (FLeu2), or 5,5′-difluoroleucine (diFLeu).
Y. J. Tan, E. H. Abdelkader, I. D. Herath, A. Maleckis, G. Otting   +4 more
doaj   +1 more source

Effect of Acute Low-load High-repetition Resistance Exercise on Protein Synthetic Signaling Pathway and Satellite Cell Activation in Skeletal Muscle of Rats [PDF]

운동과학, 2020
PURPOSE Previous studies have reported that low-load high-repetition (LLHR) resistance exercise can induce muscle hypertrophy to a similar extent as high-load low-repetition (HLLR).
Chang-Hyun Lim, Chang-Keun Kim
doaj   +1 more source