Results 151 to 160 of about 3,180,845 (188)

Quantitation of HTLV-I proviral load by a TaqMan real-time PCR assay

Journal of Virological Methods, 2002
A quantitative real-time PCR assay was developed to measure the proviral load of human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells (PBMCs). The HTLV-I copy number was referred to the actual amount of cellular DNA by means of the quantitation of the albumin gene.
Axelle, Dehée, Raymond, Césaire, Nathalie, Désiré, Agnès, Lézin, Olivier, Bourdonné, Odile, Béra, Yves, Plumelle, Didier, Smadja, Jean Claude, Nicolas   +8 more
openaire   +2 more sources

Use of synthetic oligonucleotides for determination of HTLV‐1 proviral load by real‐time PCR: a helpful alternative approach in the clinical management

Journal of Applied Microbiology, 2020
To evaluate the potential use of synthetic oligonucleotides as a standard curve for proviral load (PVL) of human T‐cell leukaemia virus type 1 (HTLV‐1) quantification in peripheral blood mononuclear cells (PBMC) of HTLV‐1‐infected individuals by ...
L. Bandeira, M. Puga, V. Paula, L. Demarchi, Gislene Lichs, J. A. Domingos, R. V. Cunha, S. Uehara, A. Motta-Castro   +8 more
semanticscholar   +1 more source

Low proviral small ruminant lentivirus load as biomarker of natural restriction in goats

Veterinary Microbiology, 2016
Small ruminant lentiviruses (SRLV) globally affect welfare and production of sheep and goats and are mainly controlled through elimination of infected animals, independently of the viral kinetics within the single animal. Control programs are based on highly sensitive serological tests, however the existence of low antibody responders leads to the ...
Helena Crespo, Luigi Bertolotti, Margherita Proffiti, Paolo Cascio, Fulvia Cerruti, Pier Luigi Acutis, Damián de Andrés, Ramsés Reina, Sergio Rosati   +8 more
openaire   +3 more sources

Quantitative PCR as a Tool to Determine the Reticuloendotheliosis Virus–Proviral Load of Fowl Poxvirus

Avian Diseases Digest, 2009
Sequences of the reticuloendotheliosis virus (REV), an avian retrovirus, are integrated into the genome of fowl poxvirus (FPV). We developed and evaluated a quantitative multiplex real-time PCR (multiplex qPCR) assay to determine the REV-proviral load of FPV strains.
R, Hauck, C, Prusas, H M, Hafez, D, Lüschow   +3 more
openaire   +2 more sources

Differences in proviral DNA load between HIV-1- and HIV-2-infected patients

AIDS, 2008
The lesser pathogenicity of HIV-2 relative to HIV-1 is generally attributed to its slower replication. To compare the amounts of total HIV DNA during human HIV-1 and HIV-2 infection, we developed a quantitative real-time PCR method with a unique external quantification standard based on a single plasmid harboring both the HIV-1 and the HIV-2 LTR.Viral ...
Marie, Gueudin, Florence, Damond, Joséphine, Braun, Audrey, Taïeb, Véronique, Lemée, Jean-Christophe, Plantier, Geneviève, Chêne, Sophie, Matheron, Françoise, Brun-Vézinet, François, Simon   +9 more
openaire   +2 more sources

Severe strongyloidiasis complicated by meningitis and hydrocephalus in an HTLV-1 carrier with increased proviral load

Journal of Infection and Chemotherapy, 2003
We report a 47-year-old Japanese man who was a human T-cell leukemia virus type 1 (HTLV-1) carrier with strongyloidiasis, and who was born in an area endemic for both Strongyloides stercoralis ( S. stercoralis) and HTLV-1. He presented with edema of both legs. Laboratory examination on admission revealed hypoalbuminemia, and S. stercoralis rhabditiform
Masao, Satoh, Atsushi, Futami, Kenichiro, Takahira, Makoto, Kodaira, Tokutaro, Tanaka, Ken, Kuriki, Eitaro, Hori   +6 more
openaire   +2 more sources

Potential applications of proviral load measurement in clinical retrovirology.

Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association, 1995
We have previously noted an association between proviral load and the severity of immune disease in individuals with a wide range of CD4 cell counts. Using the quantitative DNA polymerase chain reaction technology developed in our laboratory, we sought to extend these observations, with a view to establishing guidelines for the use of proviral load in ...
B, Conway, M, Montpetit, J, Raboud, T, Salas, D, Dufour, J S, Montaner, M V, O'Shaughnessy   +6 more
openaire   +1 more source

HIV-2-specific cytotoxic T-lymphocyte activity is inversely related to proviral load

AIDS, 1995
To characterize HIV-specific cytotoxic T-lymphocyte (CTL) activities in HIV-2-infected individuals and to relate these to HIV-2 proviral load.Peripheral blood mononuclear cells were collected from 16 HIV-2-seropositive and four HIV-1/2 dually seropositive subjects.
K, Ariyoshi, F, Cham, N, Berry, S, Jaffar, S, Sabally, T, Corrah, H, Whittle   +6 more
openaire   +2 more sources

Investigation of Proviral Load in Individuals Infected with Human T-Lymphotropic Virus Type II

AIDS Research and Human Retroviruses, 1995
Human T-lymphotrophic virus type II (HTLV-II) has not yet been associated with any disease. Little is known about the proviral loads of HTLV-II in vivo and its relationship, if any, to lack of pathogenicity. We determined the HTLV-II proviral copy number in peripheral blood lymphocyte (PBL) samples from 49 HTLV-II-infected individuals, of whom 25 were ...
T C, Woods, J M, Graber, R C, Hershow, R F, Khabbaz, J E, Kaplan, W, Heneine   +5 more
openaire   +2 more sources

Low DNA HTLV-2 proviral load among women in São Paulo City

Virus Research, 2008
HTLV-2 infections are almost always asymptomatic, and diseases associated with the infection are rarely reported. Little information is available on the relationship between HTLV-2 proviral load and gender or expression of disease, especially among patients with HIV-1 co-infection.We studied 77 HTLV-2-infected subjects followed in our clinic for the ...
Patrícia, Montanheiro, Ingrid, Olah, Ligia M I, Fukumori, Jerusa, Smid, Augusto César Penalva de, Oliveira, Luis I B, Kanzaki, Luiz Augusto M, Fonseca, Alberto J S, Duarte, Jorge, Casseb   +8 more
openaire   +2 more sources