Results 141 to 150 of about 113,972,927 (185)

Characterization of Exotoxin of Pseudomonas aeruginosa

Journal of Infectious Diseases, 1974
Abstract : Heat-labile exotoxin of Pseudomonas aeruginosa has been purified and concentrated. Studies in mice have shown that the toxin affects protein synthesis in the liver within 3 hr of administration, whereas inhibition of protein synthesis in other organs occurs only during terminal stages.
O R, Pavlovskis, L T, Callahan, R D, Meyer   +2 more
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Pseudomonas exotoxin — Immunotoxins

1988
Monoclonal antibodies can be coupled with PE to make very potent ITs. Two of these ITs (PE-HB21 and OVB-3-PE) have been shown to have antitumor activity in a nude mouse model of ovarian cancer. PE ITs are at least 10-fold more active than the corresponding RTA IT.
D J, FitzGerald, M C, Willingham, I, Pastan   +2 more
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Pseudomonas aeruginosa Exotoxin A

Journal of Burn Care & Rehabilitation, 1992
Bacterial concentrations greater than 10(5) colony-forming units/gm of tissue prevent wound healing. However, it has not been determined whether it is the number of bacteria or a toxin produced by these organisms that impedes the wound healing process.
John P. Heggers, Scott Haydon, Francis Ko, Peter G. Hayward, Martin C. Robson   +4 more
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Exotoxins of Pseudomonas aeruginosa. II. Concentration, Purification, and Characterization of Exotoxin A

Journal of Infectious Diseases, 1973
Exotoxin A of Pseudomonas aeruginosa (PA-103) could be concentrated readily by precipitation with zinc acetate and ammonium sulfate. The toxin was purified by column chromatography with use of DEAE-cellulose and Sephadex G-200. The final product contained about 8,000 mouse LD,,0/mg of protein.
P V, Liu, S, Yoshii, H, Hsieh
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Pseudomonas aeruginosaExotoxin A

New England Journal of Medicine, 1980
Pseudomonas aeruginosa is an opportunistic pathogen that causes more than 100,000 infections in the United States each year. Pseudomonas infections are associated with considerable morbidity and mortality despite the use of modern antibiotics.1 Recognition of the limitations of existing therapy for pseudomonas disease has stimulated renewed interest in
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Crystallization of exotoxin A from Pseudomonas aeruginosa

Journal of Molecular Biology, 1982
Abstract Exotoxin A from Pseudomonas aeruginosa has been crystallized in a form suitable for high resolution diffraction analysis. The crystals, grown in the presence of high concentrations of polyethylene glycol (20%, w/v) and of NaCl (1.5 m ), are monoclinic and contain one monomeric toxin molecule per asymmetric unit. The space group is P 2 1 ,
R J, Collier, D B, McKay
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Redirecting Pseudomonas exotoxin.

Seminars in cell biology, 1992
Pseudomonas exotoxin (PE) is a three-domain bacterial toxin that kills mammalian cells by gaining entry to the cytosol and inactivating protein synthesis. The pathway of toxin entry includes binding to a surface receptor, internalization via coated pits and endosomes, proteolytic processing, reduction of disulfide bonds and finally the translocation of
D, FitzGerald, I, Pastan
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The Role of Exotoxin A in Pseudomonas Disease and Immunity

Clinical Infectious Diseases, 1983
Exotoxin A is an extracellular enzyme that is produced by most clinical strains of Pseudomonas aeruginosa. It is a single-chain polypeptide (molecular weight, 71,000) with A and B fragments that mediate enzymatic and cell-binding functions, respectively.
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Pseudomonas aeruginosa exotoxin A

1997
Abstract Pseudomonas aeruginosa exotoxin A (PEA, MW 66 kDa, 613 amino acids, sequence accession number: PSEETA K01397) consists of three major domains (Allured et al. 1986), as indicated in Fig. 1. The N-terminal domain la (amino acids 1-252) binds to the ai-macroglobulin receptor at the cell surface (Kounnas et al.
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Clinical Trials with Pseudomonas Exotoxin Immunotoxins

1998
Pseudomonas exotoxin (PE) has been used to make immunotoxins for cancer therapy for more than a decade (FitzGerald et al. 1983). The function of PE is described in detail elsewhere in this book. In summary, PE is a 613 amino acid (66kDa) single-chain protein secreted by Pseudomonas aeruginosa. X-ray crystallography (Allured et al.
L H, Pai, I, Pastan
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