Results 161 to 170 of about 14,132 (182)

Mutant PTR1 Proteins from Leishmania tarentolae: Comparative Kinetic Properties and Active-Site Labeling

Archives of Biochemistry and Biophysics, 1999
PTR1, the gene promoting MTX resistance following gene amplification or DNA transfection in Leishmania tarentolae and selected mutants, has been cloned and heavily overexpressed (>100 mg/liter) in Escherichia coli strain BL21 (DE3). Protein has been purified, essentially to homogeneity, in two steps, via ammonium sulfate precipitation and ...
Chi-Feng Chang, Tom Bray, John M. Whiteley   +2 more
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Structure of pteridine reductase (PTR1) fromLeishmania tarentolae

Acta Crystallographica Section D Biological Crystallography, 2003
The protozoan parasites Leishmania utilize a pteridine-reducing enzyme, pteridine reductase (PTR1), to bypass antifolate inhibition. The crystal structure of PTR1 from L. tarentolae has been solved as a binary complex with NADPH at 2.8 A resolution. The structure was solved by molecular-replacement techniques using the recently reported L.
Haiyan, Zhao, Tom, Bray, Marc, Ouellette, Ming, Zhao, Rose Ann, Ferre, David, Matthews, John M, Whiteley, Kottayil I, Varughese   +7 more
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Crystallization of recombinant Leishmania major pteridine reductase 1 (PTR1)

Acta Crystallographica Section D Biological Crystallography, 1999
The enzyme pteridine reductase (PTR1) has recently been discovered in the protozoan parasite Leishmania and validated as a target for therapeutic intervention. PTR1 is responsible for the salvage of pteridines and also contributes to antifolate drug resistance.
Gourley, David G., Luba, James, Hardy, Larry W., Beverley, Stephen M., Hunter, William N.   +4 more
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New antileishmanial quinoline linked isatin derivatives targeting DHFR-TS and PTR1: Design, synthesis, and molecular modeling studies

European Journal of Medicinal Chemistry, 2023
In a search for new drug candidates for one of the neglected tropical diseases, leishmaniasis, twenty quinoline-isatin hybrids were synthesized and tested for their in vitro antileishmanial activity against Leishmaniamajor strain. All the synthesized compounds showed promising in vitro activity against the promastigote form in a low micromolar range ...
Ahmed, Sabt, Wagdy M, Eldehna, Tamer M, Ibrahim, Adnan A, Bekhit, Rasha Z, Batran   +4 more
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Pterin and Folate Reduction by theLeishmania tarentolaeH Locus Short-Chain Dehydrogenase/Reductase PTR1

Archives of Biochemistry and Biophysics, 1997
Overproduction of the short-chain dehydrogenase/reductase PTR1 confers resistance to the dihydrofolate reductase inhibitor methotrexate in the protozoan parasite Leishmania. Genetic analysis has previously implicated PTR1 in pterin and folate metabolism. PTR1 was purified from a fusion protein expressed in Escherichia coli. Purified PTR1 exhibits NADPH-
J, Wang, E, Leblanc, C F, Chang, B, Papadopoulou, T, Bray, J M, Whiteley, S X, Lin, M, Ouellette   +7 more
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The fission yeast ptr1+ gene involved in nuclear mRNA export encodes a putative ubiquitin ligase

Biochemical and Biophysical Research Communications, 2004
Fission yeast ptr1-1 is one of the mRNA transport mutants that accumulate poly(A)+ RNA in the nuclei at the nonpermissive temperature. We found that the ptr1+ gene encodes a homolog of Saccharomyces cerevisiae Tom1p, a hect type ubiquitin ligase. In ptr1-1, a conserved amino acid in the hect domain of Ptr1p is mutated.
Tomoko, Andoh, Abul Kalam, Azad, Asako, Shigematsu, Yasumi, Ohshima, Tokio, Tani   +4 more
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Degradation of pteridine reductase 1 (PTR1) enzyme during growth phase in the protozoan parasite Leishmania donovani

Experimental Parasitology, 2007
Pteridine reductase 1 (PTR1) is an essential enzyme of pterin and folate metabolism in the protozoan parasite Leishmania. The present work is focused on the degradation of PTR1 during growth phase in Leishmania donovani. Western blot analysis with PTR1-GFP transfected promastigotes revealed that PTR1 protein was degraded in the stationary phase of ...
Pranav, Kumar, Shyam, Sundar, Neeloo, Singh   +2 more
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RNA interference regulates the cell cycle checkpoint through the RNA export factor, Ptr1, in fission yeast

Biochemical and Biophysical Research Communications, 2012
Ago1, an effector protein of RNA interference (RNAi), regulates heterochromatin silencing and cell cycle arrest in fission yeast. However, the mechanism by which Ago1 controls cell cycle checkpoint following hydroxyurea (HU) treatment has not been elucidated.
Tetsushi, Iida, Naoko, Iida, Yasuhiro, Tsutsui, Fumiaki, Yamao, Takehiko, Kobayashi   +4 more
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Could chroman-4-one derivative be a better inhibitor of PTR1? – Reason for the identified disparity in its inhibitory potency in Trypanosoma brucei and Leishmania major

Computational Biology and Chemistry, 2020
Most notable Kinetoplastids are of the genus Trypanosoma and Leishmania, affecting several millions of humans in Africa and Latin America. Current therapeutic options are limited by several drawbacks, hence the need to develop more efficacious inhibitors.
Kehinde F. Omolabi, Emmanuel Amarachi Iwuchukwu, Paul Olalekan Odeniran, Mahmoud E. S. Soliman   +3 more
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A Deep Learning Pipeline for Generating New PTR1 Inhibitors Targeting Leishmania Disease

2025 International Conference on Machine Intelligence and Smart Innovation (ICMISI)
M Esmail Ehab, Menna S. Hany, Karen Tamer, Peter Emil, Marwa S. Hany, Tamer M. Ibrahim, Amal Aboulhassan   +6 more
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