Results 41 to 50 of about 447,036 (202)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Implementation and Application of a Generative AI Virtual Librarian

International Journal of Librarianship
Since its transformation into Taiwan’s first national digital library in 2013, the National Library of Public Information (NLPI) has continuously adopted innovative technologies to advance intelligent services.
Yun-Fan Chen, Hsiang-Ping Ma
doaj   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Developing Marketing Strategies for Public Libraries

Marketing Libraries Journal, 2018
The objective of marketing is to demonstrate the unlimited possibilities an institution has to offer to its target audience. For public libraries, this means engaging current and new users to attend programs, use resources and view the library as a ...
Monica Sanchez, Chloe R. Van Stralendorff   +1 more
doaj  

New Publishing Model for Virginia Libraries Journal

Virginia Libraries, 2018
The Virginia Libraries journal is making a change to its publication model to publish each article individually with a volume compilation on completion to make the journal’s content as accessible as possible, and to increase the efficiency of our ...
Virginia Pannabecker, Renee DiPilato, Paige Flanagan, Susan LaParo, Robin Sofge, Luke D. Vilelle   +5 more
doaj   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Inclusive Services in Public Libraries: the example of the Public Library “Fran Galović” Koprivnica concerning the social integration of people with disabilities

Čitalište
Public libraries play a key role in social inclusion, providing equal opportunities for all users, including vulnerable groups such as people with disabilities.
Danijela Petrić, Maja Krulić Gačan
doaj   +1 more source

Digital Collections in Regional Public Library “P. R. Slaveikov” – Veliko Tarnovo in the Context of the National Program for Preservation and Promotion of Literary Heritage

Digital Presentation and Preservation of Cultural and Scientific Heritage, 2011
Nowadays the great public libraries in Bulgaria are gaining the appearance of digital centers which provide new informational resources and services in the digital space.
Kalina Ivanova, Kaloyan Zdravkov
doaj   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source