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Pulsed-Field Gel Electrophoresis for Listeria monocytogenes

, 2015
Pulsed-Field Gel Electrophoresis (PFGE) is a molecular subtyping method with high discriminatory power, reproducibility, and epidemiological concordance for the subtyping of Listeria monocytogenes and other bacteria. PFGE uses rare-cutting restriction enzymes (macrorestriction) that cut the genomic DNA, usually resulting in 6-25 DNA fragments ranging ...
Laura, Luque-Sastre, Séamus, Fanning, Edward M, Fox   +2 more
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Pulsed Field Gel Electrophoresis

2003
Pulsed- field gel electrophoresis (PFGE) was first described by Schwartz and Cantor (1) It is now an umbrella term for the alternating of an electric field in more than one direction through a solid matrix to achieve the separation of DNA fragments.
A J, Hillier, B E, Davidson
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Pulsed-field gel electrophoresis

Nature Protocols, 2007
This protocol describes pulsed-field gel electrophoresis (PFGE), a method developed for separation of large DNA molecules. Whereas standard DNA gel electrophoresis commonly resolves fragments up to approximately 50 kb in size, PFGE fractionates DNA molecules up to 10 Mb.
Jill, Herschleb, Gene, Ananiev, David C, Schwartz   +2 more
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Pulsed field gel electrophoresis

Analytical Chemistry, 1991
The development of pulsed field gel electrophoresis has increased by 2 orders of magnitude the size of DNA molecules that can be routinely fractionated and analyzed. This increase is of major importance to molecular biology because it simplifies many previously laborious investigations and makes possible many new ones.
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Pulsed field sequencing gel electrophoresis

ELECTROPHORESIS, 1992
AbstractThe effect of pulsed fields on sequencing gel electrophoresis is investigated, using DNA fragment markers ranging in size from 20 to 6557 bases. For high continuous electric fields (5000 V/55 cm) band inversion is observed in which fragments larger than 4000 bases migrate faster than those of 800–1000 bases.
E, Brassard, C, Turmel, J, Noolandi
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Methods for pulsed-field gel electrophoresis

Applied Biochemistry and Biotechnology, 1993
The term pulsed-field gel electrophoresis (PFGE) is used as an acronym to indicate any technique that resolves (large) DNA molecules by continuous reorientation. It bridges the resolution gap between cytogenetic methods (> 5 Mb) and DNA analysis (< 50 kb).
J T, Den Dunnen, G J, van Ommen
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Pulsed-field gel electrophoresis

Molecular Biotechnology, 1998
Pulsed-field gel electrophoresis (PFGE) was originally developed as a technique for providing electrophoretic karyotypes of micro-organisms. Since then the technique has evolved and diversified in many new directions. This review traces the evolution of PFGE, summarizes our understanding of its theoretical basis, and provides a comprehensive ...
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Pulsed Field Gel Electrophoresis for Bifidobacterium

2015
Pulse Field Gel Electrophoresis (PFGE), unlike conventional electrophoresis, can resolve DNA fragments greater than 30 kb and is a highly discriminatory molecular typing method. Here we describe a PFGE protocol for bifidobacteria characterized by a short lysis time determined by the addition of lysis reagents to the initial cell suspension, a reduced ...
Esther, Jiménez, Marta, Gómez, Laura, Moles   +2 more
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Pulsed‐Field Gel Electrophoresis

Current Protocols in Molecular Biology, 2000
AbstractDNA molecules longer than 25 kb are poorly resolved by standard agarose gel electrophoresis. These longer molecules can be resolved using several techniques that periodically change the direction of the electric field in the gel. This unit describes the simplest and most generally useful of the pulsed‐field techniques, field inversion ...
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Pulsed Field Gel Electrophoresis for Mycobacteria

2003
Reference EPFL-CHAPTER-151283doi:10.1385/0-89603-471-2:51View record in PubMed Record created on 2010-09-07, modified on 2017-05 ...
Philipp WJ, Gordon S, Telenti A, Cole ST
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