Agarose Gel Analysis of 15–40-kb PCR Amplimers
BioTechniques, 1996 Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland +2 more
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Epidemiological analysis of methicillin-resistant Staphylococcus aureus isolates from adult patients with cystic fibrosis [PDF]
, 2006 Using pulsed-field gel electrophoresis, we genotyped 21 methicillin-resistant Staphylococcus aureus isolates from patients attending an adult cystic fibrosis unit. Eleven patients exhibited pulsotypes related to 2 locally endemic strains.
C. Coulter +6 more
core +1 more source
Development and evaluation of a novel, semiautomated Clostridium difficile typing platform [PDF]
, 2013 We describe a novel, semiautomated Clostridium difficile typing platform that is based on PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform is reproducible with minimal intra- or interassay variability.
Burnham, Carey-Ann D +6 more
core +2 more sources
Nosocomial Transmission of Group B Streptococci Proven by Positive Environmental Culture
Oman Medical Journal, 2014 Objective: Neonates usually acquire Group B streptococcal infection vertically from the maternal birth canal during delivery. In January 2010, a Group B streptococcal outbreak investigation was conducted in response to an increased number of clinical ...
Amal Al-Maani +6 more
doaj +1 more source
Streptococcus pneumoniae serotype 3 genotypes in invasive isolates from Colombia
Biomédica: revista del Instituto Nacional de Salud, 2021 Introduction: Streptococcus pneumoniae serotype 3 is an important cause of pneumonia, bacteremia, and meningitis. Objective: To establish the circulating genotypes of S.
Zonia Katerin Alarcón +3 more
doaj +1 more source
Analysis of Serpulina hyodysenteriae strain variation and its molecular epidemiology using pulsed-field gel electrophoresis [PDF]
, 1999 Pulsed-field gel electrophoresis (PFGE) was applied as a molecular typing tool for the spirochaete Serpulina hyodysenteriae, the agent of swine dysentery.
Atyeo, R.F. +2 more
core +2 more sources
Pulsed field gel electrophoresis protocol for typing of uropathogenic Escherichia coli [PDF]
فصلنامه دانشگاه علوم پزشکی جهرم, 2011 Introduction: Pulsed Field Gel Electrophoresis (PFGE) is one of the best typing methods which is highly effective in molecular epidemiological studies in different areas. All bacterial isolates are typeable by PFGE and the results are highly reproducible.
Mojtaba Anvarinejad +4 more
doaj +1 more source
Vapor nanobubble is the more reliable photothermal mechanism for inducing endosomal escape of siRNA without disturbing cell homeostasis [PDF]
, 2020 Strategies for controlled delivery of therapeutic siRNA into living cells are in high demand as endosomal escape remains the most prominent bottleneck at the intracellular level.
Barriga, Gerardo Garcia-Diaz +16 more
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Epidemiological Typing of Moraxella Catarrhalis by Pulsed Field Gel Electrophoresis
Canadian Journal of Infectious Diseases, 1995 Pulsed field gel electrophoresis (pfge) was used to compare 59 strains of Moraxella catarrhalis to evaluate pfge for the epidemiological typing of this organism.
Susan M Davison +4 more
doaj +1 more source
Biomédica: revista del Instituto Nacional de Salud, 2013 Introducción. La leptospirosis es una infección bacteriana transmitida directa o indirectamente de animales a humanos, la cual puede resultar en una enfermedad hemorrágica grave, hepática o renal y pulmonar.
Claudia M. Romero-Vivas +8 more
doaj +1 more source