Results 21 to 30 of about 300,895 (219)

Identification of best housekeeping gene for normalization in qRT-PCR data under different development stages and stress conditions in Vigna mungo

Scientific Reports
The qRT-PCR has emerged as the most sought-after local gene expression profiling technique. The absolute quantification of the mRNA is highly affected by the handling artifacts.
Sudhir Kumar Jha, Pritee Sagar, Prateek Kumar, Shivangi Gupta, Ankita Anil Chakote, Vaibhav Kumar, Kalpana Tewari, Antara Das, Meenal Rathore, Girish Prasad Dixit, Kuldeep Kumar   +10 more
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

Characterisation of the α-GOS profile of field pea and cowpea cultivars and their modulation through sustainable processing methods

Frontiers in Sustainable Food Systems
α-galactooligosaccharides (α-GOS) are a class of non-nutritional compounds present in pulses. These carbohydrates are associated with irritable bowel syndrome (IBS).
Kalpana Tewari, Vaibhav Kumar, Ashok Kumar Parihar, Sudhir Kumar Jha, Kuldeep Kumar, Rishikesh Kumar, Man Mohan Deo, Surendra Kumar Meena, Rajeev Kumar, M. Senthil Kumar, Girish Prasad Dixit   +10 more
doaj   +1 more source

A comprehensive review on grain legumes as climate‐smart crops: Challenges and prospects

Environmental Challenges, 2022
Global food production needs to be doubled by 50% by the middle of 21st century to ensure the food and nutritional security for 9 billion people explicitly in the challenging scenarios such as land degradation, water scarcity and environmental pollution.
Asik Dutta, Ankita Trivedi, Chaitanya P. Nath, Debjyoti Sen Gupta, Kali Krishna Hazra   +4 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

Biological characterization and variability of the nucleocapsid protein gene of Groundnut bud necrosis virus isolates infecting pea from India

Phytopathologia Mediterranea, 2012
A disease of pea characterized by browning in veins, leaves and stems, mostly in growing tips, and brown circular spots on pods, was recorded in four districts of Uttar Pradesh, India.
Mohammad AKRAM, NAIMUDDIN
doaj   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

Identification of stable resistant wild pigeonpea donors to multiple predominant bruchid species in India

Scientific Reports
Identifying stable-resistant sources offers an eco-friendly and sustainable insect management strategy. Sixty-eight wild pigeonpea accessions were systematically screened against three predominant bruchid species (Callosobruchus analis, C.
Revanasidda Aidbhavi, S. J. Satheesh Naik, Didendu Dutta, Jagadish Jaba, Sanjay M. Bandi, Prakash I. Gangashetty, Ashok K. Parihar, Abhishek Bohra, Mohd. Akram, Aditya Pratap, G. P. Dixit   +10 more
doaj   +1 more source

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source