Results 11 to 20 of about 1,326,906 (303)

Rapid two-step purification of a recombinant mouse Fab fragment expressed in Escherichia coli

open access: yes, 2001
We report a rapid, large-scale process for the purification of a recombinant Fab fragment specific for the tobacco mosaic virus coat protein (Fab57P). The fragment is expressed periplasmically in Escherichia coli.
Wlad, H,   +4 more
core   +7 more sources

Purification complexity without purifications [PDF]

open access: yesJournal of High Energy Physics, 2021
Abstract We generalize the Fubini-Study method for pure-state complexity to generic quantum states by taking Bures metric or quantum Fisher information metric (QFIM) on the space of density matrices as the complexity measure.
openaire   +3 more sources

Purification of Pneumolysin [PDF]

open access: yesInfection and Immunity, 1971
A procedure for the purification of pneumolysin has been developed. Its use results in a 96-fold increase in specific activity from the original crude pneumococcal extract to the final purified product. The purified material is heat-labile and is inactivated by trypsin and cholesterol; inactivation by p ...
C N, Shumway, S J, Klebanoff
openaire   +2 more sources

Advances in the expression and purification of human PARP1: A user-friendly protocol [PDF]

open access: yes, 2023
The PARP1 (Poly (ADP-ribose) polymerase 1) enzyme is essential for single and double-strand break repair in humans. Alterations affecting PARP1 activity have severe consequences for human health and are associated with pathologies like cancer, and ...
Bruno A. Salgueiro   +9 more
core   +1 more source

Purification of Erythropoietin [PDF]

open access: yesProceedings of the National Academy of Sciences, 1971
Erythropoietin, the hormone that is the primary regulator of erythrocyte production, has been purified from anemic-sheep plasma, with a purification factor of more than 1 million. Gel electrophoresis shows that the major contaminant is desialated erythropoietin.
E, Goldwasser, C K, Kung
openaire   +2 more sources

Improvements of a high-throughput protein purification process using a calcium-dependent setup

open access: yes, 2020
The Human Secretome Project aims to produce and purify all human secreted proteins as full-length. In order to enable this, a robust, gentle and effective purification process is needed, where multiple proteins can be purified in parallel.
Hanna Tegel   +11 more
core   +1 more source

Interfacial Hydrothermal Assembly of Three-Dimensional Lamellar Reduced Graphene Oxide Aerogel Membranes for Water Self-Purification

open access: yes, 2021
Energy-saving membrane separation for water purification is increasingly desired, which requires appropriate nanofiltration membranes enabling to reject undesired solutes efficiently and allows high permeation of water.
Shuang Wang (46453)   +8 more
core   +4 more sources

Protein purification with nanoparticle-enhanced crystallisation [PDF]

open access: yes, 2020
In this study, silica nanoparticle was synthesised and used to promote lysozyme crystallisation effectively against high concentrations of protein impurity (bovine serum albumin (BSA); concentration = 25.0–50.0 mg/mL vs 5.0–25.0 mg/mL for lysozyme) at 1 ...
Chen, Wenqian   +6 more
core   +1 more source

Association between hyperuricemia and acute kidney injury in critically ill patients with sepsis

open access: yesBMC Nephrology, 2023
Background Sepsis-related AKI is related to short-term mortality and poor long-term prognoses, such as chronic renal insufficiency, late development of end-stage renal disease, and long-term mortality.
Yuan-Xia Jiang   +8 more
doaj   +1 more source

Does ligation of the dorsal branch of the cephalic vein affect the patency of a distal forearm arteriovenous fistula? A randomised study

open access: yesBMC Nephrology, 2020
Background Whether ligation of the dorsal branch of the cephalic vein during the surgical establishment of the radiocephalic arteriovenous fistula (RCAVF) favorably or adversely affects the patency rate of the RCAVF remains controversial.
Hongtao Zhang   +4 more
doaj   +1 more source

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