Results 81 to 90 of about 1,178,773 (316)

The DYRK Family: A Key Regulator of Metabolic Homeostasis and Its Therapeutic Potential in Kidney Diseases

MedComm – Future Medicine
The dual‐specificity tyrosine‐regulated kinase (DYRK) family, a conserved serine/threonine kinase group, plays a pivotal role in the regulation of metabolic homeostasis by modulating glucose/lipid metabolism, oxidative stress, and calcium‐phosphorus ...
Yixuan Zhu, Pengfei Qiao, Yukun Gan, Xiushuo Fu, Xiayue Huang, Sirui Sun, Yafei Gou, Wenjie Xia, Feng Ma, Limin Liu   +9 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Purification and characterization of two different a-L-Rhamnosidases, RhaA and RhaB, from Aspergillus aculeatus

, 2001
Two proteins exhibiting -L-rhamnosidase activity, RhaA and RhaB, were identified upon fractionation and purification of a culture filtrate from Aspergillus aculeatus grown on hesperidin.
Manzanares, Paloma, Graaff, L.H., de, de Graaff, L.H., Visser, Jaap, Broeck, H.C., Graaff, Leo H. de, Manzanares, P., Visser, J., van den Broeck, Hetty C.   +8 more
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Monoclonal antibody (mAb) purification by counter current chromatography (CCC)

, 2011
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Counter current chromatography (CCC) is a form of liquid liquid chromatography, which the Brunel Institute for Bioengineering (BIB) team have developed to ...
Fernando, Samantha
core  

Proteolytic hydrolysis and purification of the LRP/alfa-2-macroglobulin receptor domain from alpha-macroglobulins

, 2007
A new, easier and efficient purification method, using Sephacryl and DEAE-Sephacel, of the C-terminal fragment of two alpha-macroglobulins, alpha(2)-M and PZP, is presented. Two larger peptides were identified for each protein as the C-terminal fragment,
Barrera, Daniel Iván, Arbeláez, Luis Fernando, Matheus, Luisa Marina, Stigbrand, Torgny,   +3 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species

, 2000
Bibliography: leaves 163-203.This work involved the characterisation of protoporphyrinogen oxidase (PPO), the penultimate enzyme in haem biosynthesis, from Bacillus subtilis, Myxococcus xanthus, and human. A defect in human PPO causes variegate porphyria,
Siziba, Kwanele Bennett
core  

Characterizing natural separation and purification during rural polluted water self-purification in the Dashi River: a multidimensional approach to DOM and microbial interactions

The self-purification capacity of rivers, a natural analog to engineered separation processes, mitigates pollutants through complex physicochemical and biological interactions.
Graham, Nigel, Sun, Kening, Xie, Jin, Yu, Wenzheng, Liu, Ting   +4 more
core   +1 more source

Development and Validation of a Risk Prediction Model for Interdialytic Hyperkalemia in Patients Undergoing Maintenance Hemodialysis

Risk Management and Healthcare Policy
Pan Wang,1,* Jun-Nan Xiao,1,* Yan-Yu Liu,2 Long Xiao,1 Jie-Li Chen,1 Yu Wang,1 Li-Hua Yang,3 Xiu-Li Feng4 1Blood Purification Centre, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510000, People’s Republic of ...
Wang P, Xiao JN, Liu YY, Xiao L, Chen JL, Wang Y, Yang LH, Feng XL   +7 more
doaj