Results 91 to 100 of about 146,626 (295)
uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins
Nature Communications, 2018 RNA-binding proteins have important roles in gene expression and regulation. Here the authors develop uvCLAP to purify proteins and determine their binding sites without the use of radioactivity.
Daniel Maticzka +4 more
doaj +1 more source
Emerging experimental and computational methods for studying redox‐regulated structural transitions
FEBS Letters, EarlyView.Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass +2 more
wiley +1 more source
Mendata: A Framework to Purify Manipulated Training Data
, 2023 Untrusted data used to train a model might have been manipulated to endow the learned model with hidden properties that the data contributor might later exploit. Data purification aims to remove such manipulations prior to training the model.
Huang, Zonghao +2 more
core
The Study of Snānavidhi: As Described in Śaivāgamas
Prachi PrajnaSnānavidhi is an important activity in contemporary life, without which one cannot purify one’s body. In Śaivāgamas, it is one of the saṃskāras by which the Ācārya and Yajamāna purify themselves. It is a must for us to purify our bodies before performing
Deviprasad Mishra
doaj
Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies. [PDF]
FEBS Open BioWe describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Janssen B +5 more
europepmc +2 more sources
FEBS Letters, EarlyView.Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source
浙江大学学报. 农业与生命科学版, 2017 Due to limited capacity of self-purification of water in ponds, accumulation of feed residue and metabolites lead to endogenous, exogenous pollution, and eutrophication. Currently, physical, chemical and biological methods are used to purify aquacultural
WEN Xu, MA Xuzhou, FAN Wei, LI Xingxing
doaj +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
FEBS Letters, EarlyView.How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source