Results 161 to 170 of about 2,825 (207)
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Reaction of the purple membrane with a carbodimide
Biochimica Et Biophysica Acta - Bioenergetics, 1979Purple membrane was reacted with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide at pH 4.5 and 8.0. At pH 4.5, the reaction yields cross-linked bacteriorhodopsin. The cross-linking is inhibited by pretreatment of the membrane with papain, or by the presence of carbohydrazide or glycine ethyl ester in the reaction mixture.
Robert Renthal
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Structure of the Purple Membrane
Nature New Biology, 1971IN the preceding article1 it has been shown that a cell membrane fragment, the purple membrane, isolated from Halobacterium halobium2 contains retinal bound in a mole ratio of 1 : 1 to a protein of molecular weight 26,000 which is the only protein present.
A E, Blaurock, W, Stoeckenius
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The topography of the purple membrane
Nature, 1979THE purple membrane of Halobacterium halobium functions as a light-driven proton pump1,2. It is about 45 A thick3 and contains a single polypeptide chain comprising about 240 amino acids4. Retinal is covalently attached to a specific lysine residue4 and has been implicated in the absorption of incident light energy2.
J E, Walker, A F, Carne, H W, Schmitt
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Charting the Surfaces of the Purple Membrane
Journal of Structural Biology, 1999The preponderance of structural data of the purple membrane from X-ray diffraction (XRD), electron crystallography (EC), and atomic force microscopy (AFM) allows us to ask questions about the structure of bacteriorhodopsin itself, as well as about the information derived from the different techniques.
Heymann JB +6 more
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Bioinspired nanoencapsulation of purple membranes
Soft Matter, 2008Embedding biological compounds, e.g. enzymes or whole cells, in solid host material seems to be a promising approach to widen their field of application far beyond the limits of natural conditions. In fields such as medicine and biotechnology, there is great interest in new methods to produce these types of composite materials in the form of micro- or ...
Andreas, Schönafinger +3 more
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Hydration dynamics of purple membranes
Faraday Discuss., 2009Protein motions occur over many decades of time, from femtoseconds to seconds and longer. Fast (ps-ns) protein motion enabled by water dynamics on the surfaces of protein molecules is generally believed to be required for biological function. The coupling between water and protein dynamics for soluble proteins in aqueous solution is relatively well ...
Douglas J, Tobias +2 more
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Melittin-regenerated purple membrane
Biochemistry (Moscow), 2009We have investigated the character of melittin-regenerated purple membrane. Adding melittin to blue membrane causes the color transition and partial regeneration of the photocycle and the proton pump. The reconstitution of bacteriorhodopsin by melittin is proved to be charge-dependent.
Yue, Zhang, Tao, Su, Kun-Sheng, Hu
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Purple membrane and purple membrane-phospholipid Langmuir-Blodgett films
Thin Solid Films, 1985Abstract Langmuir-Blodgett films containing purple membrane fragments, which consist of the integral membrane protein bacteriorhodopsin in its native bilayer, were studied in order to determine the optimal conditions for the construction of an artificial biological membrane containing purple membrane. In pure purple membrane films, the fragments were
Jay Schildkraut, Aaron Lewis
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Asymmetric Structure of the Purple Membrane
Science, 1977There is both functional and structural evidence that bacteriorhodopsin is oriented asymmetrically across the purple membrane of Halobacterium halobium . To assess the degree of asymmetry, the x-ray diffraction data from the membrane have been analyzed for possible electron-density profiles. A recent theory predicts
A E, Blaurock, G I, King
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A Novel Glycolipid and Phospholipid in the Purple Membrane
Biochemistry, 2000A novel glycolipid of mass 1935 and a phospholipid of mass 1522 are the main residual lipids (along with traces of PGP-Me, S-TGD-1, and PG) specifically associated with "delipidated" bacteriorhodopsin fractions BR I and BR II, prepared by Triton X-100 treatment of purple membrane (PM), from a genetically engineered strain (L33) of Halobacterium ...
A. CORCELLI +4 more
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