Results 171 to 180 of about 2,825 (207)

Birefringence of Purple Membranes

Journal of Colloid and Interface Science, 1994
Abstract An investigation of the electric birefringence of aqueous suspensions of purple membrane fragments was carried out. We have determined values for the permanent dipole moment ( p ∼ 10 -24 C m) and for the membrane polarizability (Δγ ∼ 10 -28 F m 2 ) of the purple membrane fragment.
Georgi S. Todorov, Stefan Kh. Sokerov, Stoyl P. Stoylov   +2 more
openaire   +1 more source

Atomic force microscopy of purple membranes

Journal of Microscopy, 1988
SUMMARYThe surface structure of purple membranes was imaged using an atomic force probe mounted in a scanning tunnelling microscope. One of the two different membrane surfaces showed protruding, disc‐shaped features forming an hexagonal lattice with about 6 nm centre to centre spacing.
D L, Worcester, R G, Miller, P J, Bryant
openaire   +2 more sources

Assembly of Purple Membranes on Polyelectrolyte Films

Langmuir, 2009
The membrane protein bacteriorhodopsin in its native membrane bound form (purple membrane) was adsorbed and incorporated into polyelectrolyte multilayered films, and adsorption was in situ monitored by optical waveguide light-mode spectroscopy.
Marie-belle, Saab, Elias, Estephan, Thierry, Cloitre, René, Legros, Frédéric J G, Cuisinier, László, Zimányi, Csilla, Gergely   +6 more
openaire   +2 more sources

Recrystallization of the purple membrane in vivo and in vitro

Journal of Molecular Biology, 1978
Abstract The formation of large patches of the purple membrane of Halobacterium halobium was investigated. It could be shown that single membrane patches fuse and reorient their protein in vivo to form a continuous crystal lattice throughout the whole area of the membrane. Experiments with isolated membranes proved that recrystallization can also
D C, Neugebauer, H P, Zingsheim, D, Oesterhelt   +2 more
openaire   +2 more sources

Biogenesis of the purple membrane of Halobacterium halobium

Biophysics of Structure and Mechanism, 1977
A protein closely resembling the purple membrane protein pre-exists in the cell membrane of H. halobium prior to the appearance of functional bacteriorhodopsin. It is associated with a differentiated membranous structure which has been isolated on a sucrose gradient and appears to be a precursor of the purple membrane.
A, Danon, M, Brith-Lindner, S R, Caplan
exaly   +3 more sources

Crystallization of purple membrane in three dimensions

Journal of Molecular Biology, 1980
Abstract Large aggregates of purple membrane have been produced by solubilization of purple membrane in Triton X100, followed by removal of the detergent by dialysis against 0.9 m or 1.0 m -phosphate (pH 5.0). Although the crystals are not yet large enough for X-ray analysis, they appear microscopically crystalline in three dimensions, with space ...
R, Henderson, D, Shotton
openaire   +2 more sources

Bacteriorhodopsin depleted of purple membrane lipids

Biochemical and Biophysical Research Communications, 1976
Summary Purple membrane of Halobacterium halobium is partially disrupted by treatment with 20 mM dodecyl trimethyl ammonium bromide at pH 3.0. Subsequent sucrose gradient centrifugation or gel-filtration separates ⩾ 90% of the endogenous polar lipids from bacteriorhodopsin.
M, Happe, P, Overath
openaire   +2 more sources

Pressure effects on the photocycle of purple membrane

Biochemistry, 1984
We studied the effects of hydrostatic pressure on the kinetics of the photocycle of purple membrane from Halobacterium halobium. The data were interpreted in terms of a unidirectional and unbranched model. We found that all of the distinct processes of the photocycle are retarded by pressure, with the earlier, fast processes showing less sensitivity to
J, Marque, L, Eisenstein
openaire   +2 more sources

Resonance Raman studies of the purple membrane

Biochemistry, 1977
The individual resonance Raman spectra of the PM568 and M412 forms of light-adapted purple membrane from Halobacterium halobium have been measured using the newly developed flow technique. For comparison purposes, the Raman spectra of the model chromophores, all-trans- and 13-cis retinal n-butylamine, both as protonated and unprotonated Schiff bases ...
B, Aton, A G, Doukas, R H, Callender, B, Becher, T G, Ebrey   +4 more
openaire   +2 more sources

Calcium binding to the purple membrane: A molecular dynamics study

Proteins: Structure, Function, and Bioinformatics, 2008
AbstractThe purple membrane (PM) is a specialized membrane patch found in halophilic archaea, containing the photoreceptor bacteriorhodopsin (bR). It is long known that calcium ions bind to the PM, but their position and role remain elusive to date. Molecular dynamics simulations in conjunction with a highly detailed model of the PM have been used to ...
Wassenaar, Tsjerk A., Daura, Xavier, Padros, Esteve, Mark, Alan E.   +3 more
openaire   +6 more sources