Results 41 to 50 of about 69 (69)

Activation of pyridoxal kinase by metallothionein

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1989
Brain pyridoxal kinase, which uses ATP complexed to either Zn(II) or Co(II) as substrates, displays high catalytic activity in the presence of Zn-thionein and Co-thionein. Several steps intervene in the process of pyridoxal kinase activation, i.e., binding of Zn ions to ATP and interaction between Zn-ATP and the enzyme. Equilibrium binding studies show
Glen M. Scholz, Francis Kwok, Jorge E. Churchich   +2 more
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Inhibition of Pyridoxal Kinase by Methylxanthines

Enzyme, 1990
In the presence of saturating concentrations of adenosine triphosphate (ATP) and rate-limiting amounts of pyridoxal, theophylline was found to inhibit sheep brain pyridoxal kinase (EC 2.7.1.35) competitively. The apparent inhibition constant (Ki) of theophylline for pyridoxal kinase was determined as 8.7 mumol/l. Theophylline concentrations of up to 60
S. Bissbort, Johan B. Ubbink, Delport R, Vermaak Wj   +3 more
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Pyridoxal Kinase

Annals of the New York Academy of Sciences, 1990
Chymotryptic digestion of sheep brain pyridoxal kinase, a dimer of identical subunits each of 40 kDa, yields two fragments of 24 and 16 kDa with concomitant loss of catalytic activity. These fragments were separated by HPLC and used for binding studies with ATP and pyridoxal analogues.
Jorge E. Churchich, Young Kim
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The interactions between biogenic amines and pyridoxal, pyridoxal phosphate, and pyridoxal kinase

Archives of Biochemistry and Biophysics, 1972
Abstract The effect of biogenic amines on pyridoxal kinase activity has been investigated in vitro . Experiments with a purified beef brain pyridoxal kinase indicate that 3,4-dihydroxyphenylalanine (dopa), dopamine, norepinephrine, and serotonin inhibit the reaction, while tyrosine, tryptophan, and 5-hydroxytryptophan (5-HTP) have no effect. However,
R.L. Meneely, Warren F. Diven, M.R. Grever, J.T. Neary   +3 more
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On the mechanism of Escherichia coli pyridoxal kinase inhibition by pyridoxal and pyridoxal 5′-phosphate

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2015
Pyridoxal 5'-phosphate (PLP), the catalytically active form of vitamin B6, plays a crucial role in several cellular processes. In most organisms, PLP is recycled from nutrients and degraded B6-enzymes in a salvage pathway that involves pyridoxal kinase (PLK), pyridoxine phosphate oxidase and phosphatase activities.
DI SALVO, Martino Luigi, Nogués I, PARRONI, ALESSIA, Tramonti A, MILANO, TERESA, PASCARELLA, Stefano, CONTESTABILE, Roberto   +6 more
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Crystal structure of human pyridoxal kinase

Journal of Structural Biology, 2006
Pyridoxal kinase, a member of the ribokinase superfamily, catalyzes the ATP-dependent phosphorylation reaction of vitamin B6 and is an essential enzyme in the formation of pyridoxal-5'-phosphate, a key cofactor for over 100 enzymes. Pyridoxal kinase is thus regarded as a potential target for pharmacological agents.
Tao Jiang, Yun Chung Leung, Lin Tang, Peng Cao, Yong Gong   +4 more
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Pyridoxal kinase immunoreactivity in rabbit brain

Neurochemical Research, 1994
Murine polyclonal antibody against purified bovine brain pyridoxal kinase (EC 2.7.1.35) was generated and showed cross-reactivity with rabbit brain pyridoxal kinase. This antibody was used to immunohistochemically examine the distribution of pyridoxal kinase in the rabbit brain.
Koji Takada, Kensuke Joh, Tae Hirakawa-Sakurai, Kivoshi Ohkawa, Tadashi Asakura, Makoto Matsuda   +5 more
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Interaction between Pyridoxal Kinase and Pyridoxal-5-phosphate-Dependent Enzymes

Journal of Biochemistry, 2003
The interactions of two pyridoxal-5-phosphate (PLP)-dependent enzymes, alanine aminotransferase (ALT) and glutamate decarboxylase (GAD), with pyridoxal kinase (PK) were studied by fluorescence polarization as well as surface plasmon resonance techniques. The results demonstrated that PK can specifically bind to ALT and GAD.
Pik Yuen Cheung, Man Sau Wong, Yun Chung Leung, Mengsu Yang, Wan Chuen Lam, Kang To Ng, Chun Wai Tsang, Chi Chun Fong   +7 more
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INTERRELATIONSHIPS BETWEEN PYRIDOXAL PHOSPHATE AND PYRIDOXAL KINASE IN RABBIT BRAIN¹

Journal of Neurochemistry, 1970
Abstract—An inverse relationship was demonstrable between the concentration of pyridoxal phosphate and the activity of pyridoxal kinase in rabbit brain. The administration of pyridoxine elevated the concentration of pyridoxal phosphate and decreased the activity of pyridoxal kinase.
R. B. Kugel, M. S. Ebadi, E. E. McCoy
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Increased activity of pyridoxal kinase in tongue in Down's syndrome [PDF]

Journal of Intellectual Disability Research, 1991
ABSTRACT. The concentrations of B6 vitamins, and the activities of pyridoxal kinase, pyridoxamine phosphate oxidase and pyridoxal phosphate phosphatase were measured in tongue. Pyridoxal kinase activity was significantly greater (P<0.01) in Down's syndrome subjects compared with controls.
Stephen P. Coburn, W E Schaltenbrand, J D Mahuren   +2 more
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