Results 31 to 40 of about 4,001 (87)
Structure and Reaction Mechanism of Pyrrolysine Synthase (PylD)
, 2013 The final step in the biosynthesis of the 22nd genetically encoded amino acid, pyrrolysine, is catalyzed by PylD, a structurally and mechanistically unique dehydrogenase.
Beck, Philipp +3 more
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Engineering Pyrrolysine Systems for Genetic Code Expansion and Reprogramming. [PDF]
Over the past 16 years, genetic code expansion and reprogramming in living organisms has been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA synthetase (PylRS)/tRNAPyl pairs.
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, 2020 The chemical and electronic structures of the 21st and 22nd proteinogenic amino acid selenocysteine (Sec), pyrrolysine (Pyl), and their derivatives (deprotonated and protonated ions) were extensively characterized for the first time. Through the fragment
Jun Jiang +11 more
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, 2017 Pyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea
Alam, Intikhab +4 more
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Pyrrolysine, the 22nd amino acid encoded by the natural genetic code, is essential for methanogenic archaea to catabolize methylamines into methane. The structure of pyrrolysine consists of a methylated pyrroline carboxylate that is linked to the ε-amino
Lizhi Tao (1543519) +2 more
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Crystal Structure of Methylornithine Synthase (PylB): Insights into the Pyrrolysine Biosynthesis
, 2012 Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB.
List, Anja +4 more
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, 2019 Cell-free protein synthesis (CFPS) is a fast and convenient way to synthesize proteins for analytical studies and applications. CFPS, when equipped with a suitable orthogonal pair, allows for protein-site-directed labeling with desired functionalities ...
Helmut Merk (495340) +2 more
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Live Cell Imaging of Bioorthogonally Labelled Proteins Generated With a Single Pyrrolysine tRNA Gene
, 2017 Genetic code expansion enables the incorporation of non-canonical amino acids (ncAAs) into expressed proteins. ncAAs are usually encoded by a stop codon that is decoded by an exogenous orthogonal aminoacyl tRNA synthetase and its cognate suppressor tRNA,
Noa Aloush +10 more
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Atypical archaeal tRNA pyrrolysine transcript behaves towards EF-Tu as a typical elongator tRNA
, 2003 The newly discovered tRNAPyl is involved in speci®c incorporation of pyrrolysine in the active site of methylamine methyltransferases in the archaeon Methanosarcina barkeri.
Richard Giegeâ +3 more
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