Results 11 to 20 of about 4,065 (135)

Pyrrolysine-Inspired in Cellulo Synthesis of an Unnatural Amino Acid for Facile Macrocyclization of Proteins [PDF]

J Am Chem Soc, 2023
Macrocyclization has been touted as an effective strategy to enhance the in vivo stability and efficacy of protein therapeutics. Herein, we describe a scalable and robust system based on the endogenous biosynthesis of a noncanonical amino acid coupled to
Wai Shan Chan (15358961), Jiahui Cheng (6056966), Marianne M. Lee (8861957), Michael K. Chan (747667), Yuk Hei Chan (15358964), Lin Wang (11986), Jingxuan Tai (15358958)   +6 more
core   +4 more sources

Methanogenesis from tetramethylammonium and choline in Methanococcoides methylutens Q3c requires a nonpyrrolysine monomethylamine methyltransferase homolog [PDF]

Frontiers in Microbiology
Methanogenesis is a key biological process contributing to both global climate change and renewable energy via methane. Methylotrophic methanogenesis is an important, but often underappreciated, component of this process.
Jyoti Kashyap, Debdeep Das, Tomislav Ticak, Adam J. Creighbaum, Rakhsha Khatri, Donald J. Ferguson, Donald J. Ferguson   +6 more
doaj   +2 more sources

In Vivo Biosynthesis and Direct Incorporation of Noncanonical Amino Acids into Proteins [PDF]

ChemBioChem, Volume 26, Issue 22, November 17, 2025.
This review evaluates engineered (semi)autonomous cell systems for the biosynthesis and incorporation of noncanonical amino acids (ncAAs) into proteins. While semi‐autonomous cells convert supplied precursors into ncAAs autonomous cells integrate biosynthetic pathways that produce these building blocks intracellularly.
Jan Hendrik Illies, Tim Moritz Weber, Ivana Drienovská   +2 more
wiley   +2 more sources

Facile Synthesis of H‐L‐Photo‐Lysine and Its Genetic Incorporation Into Proteins [PDF]

ChemBioChem, Volume 27, Issue 10, 27 May 2026.
An efficient synthesis of a diazirine‐based noncanonical amino acid (ncAA), photo‐lysine (photoK), is reported, and its genetic incorporation into green fluorescent protein (GFP) is demonstrated. PhotoK‐enabled crosslinking is used to capture substrates and interaction partners of SIRT6 in vitro, in Escherichia coli, and in HEK293 cell lysates ...
Abu Hamza, Chiranjeev Sharma, Yana Cen
wiley   +2 more sources

A Genetically Encoded Homocysteine Precursor to Probe Protein Active Sites and to Addict Escherichia coli to a Noncanonical Amino Acid Directly Involved in Catalysis [PDF]

Angewandte Chemie, Volume 137, Issue 36, September 1, 2025.
A new genetically encoded amino acid is chemically converted into homocysteine (Hcy) under mild conditions to probe and to activate protein active sites. Surprisingly, Hcy can replace cysteine or serine residues in several enzymes. Its potential for a widespread use in proteins is further underlined by rendering Escherichia coli dependent on Hcy ...
Clara Dunker, Henning D. Mootz
wiley   +3 more sources

Rational design of a covalent ACE2 decoy receptor that broadly neutralizes SARS‐CoV‐2 variants [PDF]

Protein Science, Volume 34, Issue 10, October 2025.
Abstract The ongoing evolution of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and the emergence of variants that evade existing vaccines and antibody therapies necessitate novel, potent, and broad‐spectrum antiviral strategies. Enhancing therapeutic proteins with additional covalent binding capabilities, such as minibinders and ...
Nobumasa Hino, Risa Takada, Kyosuke Suzuki, Nagisa Tokunoh, Tatsuya Karaki, Kazumasa Ohtake, Haruna Ogami, Takafumi Nishiura, Kenji Ishimoto, Tomohito Tsukamoto, Yukio Ago, Yoshiaki Okada, Toru Okamoto, Chikako Ono, Yoshiharu Matsuura, Satoshi Obika, Kensaku Sakamoto, Yasuo Yoshioka, Shinsaku Nakagawa   +18 more
wiley   +2 more sources

Peptides as a Therapeutic Alternative Against Leishmaniasis: A Scoping Review [PDF]

Chemical Biology &Drug Design, Volume 107, Issue 5, May 2026.
This scoping review of 79 studies identified 231 antimicrobial peptides with anti‐Leishmania activity, including natural‐derived and synthetic peptides (inspired by natural sources or developed through rational design). Most were evaluated in vitro against promastigote and/or amastigote forms, with synthetic peptides showing a more promising effect ...
Maria Eduarda da Veiga Oliveira, Ellen Dyminski Parente Ribeiro, Otávio Henrique Pradi Guenther, Fernanda Tomiotto‐Pellissier   +3 more
wiley   +2 more sources

Efficient suppression of premature termination codons with alanine by engineered chimeric pyrrolysine tRNAs [PDF]

Nucleic Acids Res
Mutations that introduce premature termination codons (PTCs) within protein-coding genes are associated with incurable and severe genetic diseases.
Beaudoin, Jean-Denis, Tharp, Jeffery M., Specht, Caitlin, Dawodu, Olabode, Awawdeh, Aya, Tapia, Alejandro, Gaier, Sarah A., Vargas-Rodriguez, Oscar, Alshawi, Sarah A.   +8 more
core   +2 more sources

Transcript Regulation of the Recoded Archaeal α-l-Fucosidase In Vivo

Molecules, 2021
Genetic decoding is flexible, due to programmed deviation of the ribosomes from standard translational rules, globally termed “recoding”. In Archaea, recoding has been unequivocally determined only for termination codon readthrough events that regulate ...
Federica De Lise, Roberta Iacono, Andrea Strazzulli, Rosa Giglio, Nicola Curci, Luisa Maurelli, Rosario Avino, Antonio Carandente, Stefano Caliro, Alessandra Tortora, Fabio Lorenzini, Paola Di Donato, Marco Moracci, Beatrice Cobucci-Ponzano   +13 more
doaj   +1 more source

Studies on Pyrrolysine - The 22nd Amino Acid

, 2010
Recently, a 22nd amino acid has been discovered, pyrrolysine. Pyrrolysine (pyl) is coded by the translation of a amber (UAG) codon. Pyl was first found in the active site for genes encoding for methylamine methyltransferases in Methanosarcinaceae.
Thongkham, Jaylong Wong
core   +4 more sources