Results 1 to 10 of about 4,065 (135)

Insights into pyrrolysine function from structures of a trimethylamine methyltransferase and its corrinoid protein complex [PDF]

open access: yesCommunications Biology, 2023
Structures of Methanosarcina barkeri trimethylamine methyltransferase (MttB) with its substrates reveal the role of pyrrolysine in methyl group transfer from trimethylamine to the corrinoid cofactor in MttC.
Jiaxin Li   +6 more
doaj   +3 more sources

Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway

open access: yesNature Communications, 2021
Pyrrolysine (Pyl) exists in nature as the 22nd proteinogenic amino acid, but studies of Pyl have been hindered by the difficulty and inefficiency of both its chemical and biological syntheses.
Joanne M. L. Ho   +4 more
doaj   +2 more sources

Programmed Deviations of Ribosomes From Standard Decoding in Archaea

open access: yesFrontiers in Microbiology, 2021
Genetic code decoding, initially considered to be universal and immutable, is now known to be flexible. In fact, in specific genes, ribosomes deviate from the standard translational rules in a programmed way, a phenomenon globally termed recoding ...
Federica De Lise   +12 more
doaj   +1 more source

An Ecological Basis for Dual Genetic Code Expansion in Marine Deltaproteobacteria

open access: yesFrontiers in Microbiology, 2021
Marine benthic environments may be shaped by anthropogenic and other localized events, leading to changes in microbial community composition evident decades after a disturbance.
Veronika Kivenson   +2 more
doaj   +1 more source

Unconventional genetic code systems in archaea

open access: yesFrontiers in Microbiology, 2022
Archaea constitute the third domain of life, distinct from bacteria and eukaryotes given their ability to tolerate extreme environments. To survive these harsh conditions, certain archaeal lineages possess unique genetic code systems to encode either ...
Kexin Meng   +4 more
doaj   +1 more source

Virtual 2D map of cyanobacterial proteomes

open access: yesPLoS ONE, 2022
Cyanobacteria are prokaryotic Gram-negative organisms prevalent in nearly all habitats. A detailed proteomics study of Cyanobacteria has not been conducted despite extensive study of their genome sequences.
Tapan Kumar Mohanta   +4 more
doaj   +2 more sources

Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery

open access: yesCell Reports: Methods, 2023
Summary: Stop codon suppression using dedicated tRNA/aminoacyl-tRNA synthetase (aaRS) pairs allows for genetically encoded, site-specific incorporation of non-canonical amino acids (ncAAs) as chemical handles for protein labeling and modification.
Birthe Meineke   +7 more
doaj   +1 more source

Synthesis of spiropyrrolizidines containing quinoxaline and pyrrole fragments [PDF]

open access: yesИзвестия Саратовского университета. Новая серия: Серия Химия. Биология. Экология, 2023
The reaction of 1,3-dipolar cycloaddition azomethine ylides obtained by the in situ interaction of 11H-indeno[1,2-b]quinoxalin-11-one and proline, and 3-phenyl-1-pyrrolyl-2-en-1-ones has been used for the synthesis of substituted spiropyrrolizidines in ...
Borisova, Svetlana Vasilievna   +2 more
doaj   +1 more source

Tuning phenylalanine fluorination to assess aromatic contributions to protein function and stability in cells

open access: yesNature Communications, 2023
The aromatic side-chains of phenylalanine, tyrosine, and tryptophan interact with their environments via both hydrophobic and electrostatic interactions.
Grace D. Galles   +11 more
doaj   +1 more source

Anticodon table of the chloroplast genome and identification of putative quadruplet anticodons in chloroplast tRNAs

open access: yesScientific Reports, 2023
The chloroplast genome of 5959 species was analyzed to construct the anticodon table of the chloroplast genome. Analysis of the chloroplast transfer ribonucleic acid (tRNA) revealed the presence of a putative quadruplet anticodon containing tRNAs in the ...
Tapan Kumar Mohanta   +2 more
doaj   +1 more source

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