Results 261 to 270 of about 149,531 (302)
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Relaxation spectra of pyruvate kinase
Biochimica et Biophysica Acta (BBA) - Enzymology, 1970Relaxation studies using the temperature jump have been carried out on pyruvate kinase (ATP: pyruvate phosphotransferase, EC 2.7.1.40) isolated from rabbit muscle. Several concentration-independent relaxation effects with associated relaxation times of approx.
G G, Hammes, J, Simplicio
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Insulin: Inducer of Pyruvate Kinase
Science, 1965The hepatic pyruvate kinase activity markedly decreased when rats were made diabetic by alloxan injection. Insulin treatment induced new synthesis of pyruvate kinase which was prevented by injection of ethionine and actinomycin D. The evidence indicated that the increased pyruvate kinase activity induced by insulin entails at a certain stage a ...
G, WEBER, N B, STAMM, E A, FISHER
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Radiotracer assay of pyruvate kinase
Analytical Biochemistry, 1971Abstract A radiotracer enzyme assay for the determination of pyruvate kinase activity is described. The rate of the enzyme catalyzed reaction is determined by radioassay of either ATP- 3 H formed or unreacted ADP- 3 H, or both after their separations from the assay reaction mixture by TLC.
J F, Morningstar, D G, Therriault
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Pyruvate kinase of higher plants
Biochimica et Biophysica Acta (BBA) - General Subjects, 1973Abstract 1. 1. Pyruvate kinase (EC 2.7.1.40) was extracted from pea seeds and carrots and partially purified by fractionation with (NH4)2SO4. 2. 2. The enzyme from both sources showed hyperbolic kinetics for the substrates phosphoenolpyruvate and ADP. Mg2+ or Mn2+ and a movement cation were required for enzyme activity.
J D, Tomlinson, J F, Turner
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Pyruvate Kinase Deficiency and Malaria
New England Journal of Medicine, 2008Malaria that is caused by Plasmodium falciparum is a significant global health problem. Genetic characteristics of the host influence the severity of disease and the ultimate outcome of infection, and there is evidence of coevolution of the plasmodium parasite with its host.
Kodjo, Ayi +7 more
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Turnover of liver pyruvate kinase
Archives of Biochemistry and Biophysics, 1980Abstract The relative rates of synthesis and degradation for liver pyruvate kinase have been determined in rats fed standard lab chow, fasted, and refed a high-carbohydrate-low-protein diet. Relative rates of synthesis and apparent rates of degradation were determined by pulse-labeling the enzyme in vivo with l -[4,5- 3 H]leucine and by measuring ...
C, Cladaras, G L, Cottam
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Pyruvate kinase of Brevibacterium liquefaciens
Archives of Biochemistry and Biophysics, 1970Abstract Pyruvate kinase was purified 270-fold from an extract of Brevibacterium liquefaciens and its regulatory properties were examined. The purified enzyme existed as an almost inactive form. Preincubation with ATP, NaCl, or with KCl caused marked activation of the enzyme, although these compounds were inhibitory if added during the reaction.
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The inhibition of pyruvate kinase by ATP
Biochemical and Biophysical Research Communications, 1968Abstract The inhibition of pyruvate kinase by ATP was investigated under various conditions. It was concluded that the inhibition reported by previous workers was attributable to the magnesium complexing properties of ATP and it was shown that the inhibition was abolished completely when sufficient magnesium was added.
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Pyruvate Kinase in Human Erythrocytes
Scandinavian Journal of Haematology, 1969The importance of efficient separation of erythrocytes from leucocytes when studying haemolysates is shown experimentally, pyruvate kinase determination given as an example. The use of the spiral centrifuge is recommended.
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Mitapivat, a novel pyruvate kinase activator, for the treatment of hereditary hemolytic anemias
Therapeutic Advances in Hematology, 2021Hanny Al-Samkari, Eduard J Van Beers
exaly