Results 301 to 310 of about 237,828 (335)

The stereochemistry of pyruvate kinase

Biochemical and Biophysical Research Communications, 1970
Abstract α-Ketobutyrate enol phosphate consisting of approximately 80% cis and 20% trans isomers was prepared by the Perkow reaction. It was converted by pyruvate kinase in 2H2O, to α-ketobutyrate which on oxidative decarboxylation with hydrogen peroxide gave largely (2 R )-2- 2 H-propionate containing one atom of deuterium ...
W E, Bondinell, D B, Sprinson
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Mutations in pyruvate kinase

Human Mutation, 1996
Pyruvate kinase (PK) deficiency due to mutations of the PKLR gene is a common cause of hereditary nonspherocytic hemolytic anemia. Thus far, 55 different mutations have been described in patients with PK-deficient hemolytic anemia. Polymorphisms within the PKLR gene and in the tightly linked glucocerebrosidase (GBA) gene suggest that PK deficiency may ...
E, Beutler, L, Baronciani
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Pyruvate kinase electrophoresis in normal and pyruvate kinase deficient hemolysates

Clinica Chimica Acta, 1974
Abstract Two bands of pyruvate kinase activity were demonstrated in normal hemolysates with high voltage electrophoresis at pH 5.3 using cellulose acetate strips. Heterozygotes with red cell pyruvate kinase deficiency also exhibited two bands. Six of ten homozygous patients showed two bands, the remaining four only one band.
B, Wonneberger, W, Schröter
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Pyruvate kinase deficiency in children

Pediatric Blood & Cancer, 2021
AbstractBackgroundPyruvate kinase deficiency (PKD) is a rare, autosomal recessive red blood cell enzyme disorder, which leads to lifelong hemolytic anemia and associated complications from the disease and its management.MethodsAn international, multicenter registry enrolled 124 individuals younger than 18 years old with molecularly confirmed PKD from ...
Satheesh Chonat, Stefan W. Eber, Susanne Holzhauer, Nina Kollmar, D. Holmes Morton, Bertil Glader, Ellis J. Neufeld, Hassan M. Yaish, Jennifer A. Rothman, Mukta Sharma, Yaddanapudi Ravindranath, Heng Wang, Vicky R. Breakey, Sujit Sheth, Heather A. Bradeen, Hasan Al‐Sayegh, Wendy B. London, Rachael F. Grace   +17 more
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Regulation of pyruvate kinase

Advances in Enzyme Regulation, 1969
Abstract The mechanism and sequence of events of the induction of liver pyruvate kinase by sucrose and fructose administration were examined. Actinomycin and cycloheximide prevented the sucrose and fructose induced rise in pyruvate kinase activity.
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Defective erythrocyte pyruvate kinase

Clinica Chimica Acta, 1976
A defective pyruvate kinase (EC 2.7.1.40) is described. The abnormal PK is characterized by a shift in the R in equilibrium T equilibrium to the T-state. The Ko.5 for the substrate phosphoenol pyruvate is about 6 times higher than for the normal enzyme, while the KM value for the positive effector Fru-1, 6-P2 is increased.
G E, Staal, R P, Ceerdink, A M, Vlug, M L, Hamelink   +3 more
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Pyruvate kinase of Bacillus subtilis

Biochimica et Biophysica Acta (BBA) - Enzymology, 1972
Abstract Conditions are reported which stabilize pyruvate kinase (ATP: pyruvate phosphotransferase, EC 2.7.1.40) activity in extracts of Bacillus subtilis. These conditions include high protein concentrations and the presence of KCl and phosphoenolpyruvate.
M, Diesterhaft, E, Freese
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Relaxation spectra of pyruvate kinase

Biochimica et Biophysica Acta (BBA) - Enzymology, 1970
Relaxation studies using the temperature jump have been carried out on pyruvate kinase (ATP: pyruvate phosphotransferase, EC 2.7.1.40) isolated from rabbit muscle. Several concentration-independent relaxation effects with associated relaxation times of approx.
G G, Hammes, J, Simplicio
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Pyruvate kinase of higher plants

Biochimica et Biophysica Acta (BBA) - General Subjects, 1973
Abstract 1. 1. Pyruvate kinase (EC 2.7.1.40) was extracted from pea seeds and carrots and partially purified by fractionation with (NH4)2SO4. 2. 2. The enzyme from both sources showed hyperbolic kinetics for the substrates phosphoenolpyruvate and ADP. Mg2+ or Mn2+ and a movement cation were required for enzyme activity.
J D, Tomlinson, J F, Turner
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Radiotracer assay of pyruvate kinase

Analytical Biochemistry, 1971
Abstract A radiotracer enzyme assay for the determination of pyruvate kinase activity is described. The rate of the enzyme catalyzed reaction is determined by radioassay of either ATP- 3 H formed or unreacted ADP- 3 H, or both after their separations from the assay reaction mixture by TLC.
J F, Morningstar, D G, Therriault
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