The Contribution of QF-PCR and Pathology Studies in the Diagnosis of Diandric Triploidy/Partial Mole [PDF]
Diagnostics, 2021 Objective: the aim of our study was to assess the contribution of quantitative fluorescent polymerase chain reaction (QF-PCR) and pathology studies in the diagnosis of diandric triploidies/partial hydatidiform moles.
Leticia Benítez +6 more
doaj +2 more sources
Validation of QF-PCR for prenatal diagnoses in a Brazilian population [PDF]
Clinics, 2017 OBJECTIVES: Quantitative fluorescence polymerase chain reaction (QF-PCR) is a rapid and reliable method for screening aneuploidies, but in Brazil, it is not used in public services.
Renata Wendel de Moraes +6 more
doaj +2 more sources
Comparison of the combined use of CNV-seq and karyotyping or QF-PCR in prenatal diagnosis: a retrospective study [PDF]
Scientific Reports, 2023 To elevate the accuracy of diagnostic results, CNV-seq is usually performed simultaneously with karyotyping or QF-PCR. Although several studies have investigated the performance of the combined use of CNV-seq with karyotyping or QF-PCR, there have been ...
Hao Zhang +6 more
doaj +2 more sources
Usefulness of combined NGS and QF‐PCR analysis for product of conception karyotyping [PDF]
Reproductive Medicine and Biology, 2022 Purpose Since chromosomal abnormalities can be detected in more than half of miscarriages, cytogenetic testing of the product of conception (POC) can provide important information when preparing for a subsequent pregnancy. Conventional karyotyping is the
Takema Kato +14 more
doaj +2 more sources
Improving prenatal diagnosis with combined karyotyping, CNV-seq and QF-PCR: a comprehensive analysis of chromosomal abnormalities in high-risk pregnancies [PDF]
Frontiers in GeneticsObjectiveThis study aims to assess the diagnostic efficacy of a combined approach integrating chromosomal karyotyping, copy number variation sequencing (CNV-seq), and quantitative fluorescence polymerase chain reaction (QF-PCR) in detecting chromosomal ...
Jia-pei Liu +3 more
doaj +2 more sources
Combination of QF‐PCR and aCGH is an efficient diagnostic strategy for the detection of chromosome aberrations in recurrent miscarriage [PDF]
Molecular Genetics & Genomic Medicine, 2019 Background Our aim was to conduct a comprehensive genetic evaluation using the combination of QF‐PCR (quantitative fluorescence polymerase chain reaction) and aCGH (array comparative genomic hybridization) for the detection of the frequency and type of ...
Luca Lovrečić +4 more
doaj +2 more sources
Efficient and cost-effective genetic analysis of products of conception and fetal tissues using a QF-PCR/array CGH strategy; five years of data [PDF]
Molecular Cytogenetics, 2017 Background Traditional testing of miscarriage products involved culture of tissue followed by G-banded chromosome analysis; this approach has a high failure rate, is labour intensive and has a resolution of around 10 Mb.
Celia Donaghue +5 more
doaj +2 more sources
Multilevel regression modeling for aneuploidy classification and physical separation of maternal cell contamination facilitates the QF-PCR based analysis of common fetal aneuploidies. [PDF]
PLoS ONE, 2019 BackgroundThe quantitative fluorescent polymerase chain reaction (QF-PCR) has proven to be a reliable method for detection of common fetal chromosomal aneuploidies.
Predrag Noveski +5 more
doaj +2 more sources
The value of quantitative fluorescence polymerase chain reaction for the products of conception in the era of copy number variation sequencing [PDF]
Frontiers in GeneticsPurposeThe purpose of this research was to assess the effectiveness of copy number variation sequencing (CNV-Seq) and quantitative fluorescence polymerase chain reaction (QF-PCR) in detecting chromosomal abnormalities in products of conception of women ...
Shaozhe Yang +10 more
doaj +2 more sources
Effective detection of 148 cases chromosomal mosaicism by karyotyping, chromosomal microarray analysis and QF-PCR in 32,967 prenatal diagnoses [PDF]
BMC Medical GenomicsBackground Detection of mosaicism has always been difficult in prenatal diagnosis, which is to assess the value of karyotyping combined with three different molecular genetic tests for prenatal diagnosis. Retrospective review of chromosomal mosaicism (CM)
Yi Deng +9 more
doaj +2 more sources