Results 21 to 30 of about 5,809 (168)

Establishment of a 10-Plex Quantitative Fluorescent-PCR Assay for rapid diagnosis of sex chromosome aneuploidies. [PDF]

PLoS ONE, 2014
Sex chromosome aneuploidies occur commonly in the general population, with an incidence of 1 in 400 newborns. However, no tests specifically targeting sex chromosomes have been carried out in prenatal diagnosis or newborn screening, resulting in late ...
Xingmei Xie, Qiaoyi Liang
doaj   +1 more source

Detection of Fetal Aneuploidies by QF-PCR in Transcervical Cell Samples [PDF]

ISRN Genetics, 2013
Objective. To evaluate the accuracy in the diagnosis of aneuploidies of a quantitative fluorescent polymerase chain reaction (QF-PCR) assay on trophoblastic cells recovered from transcervical cells samples (TCCs) collected by intrauterine lavage (IUL). Study Design. DNA analysis was performed on cells of seemingly trophoblastic origin isolated from IUL
Riccardo Cioni, Cecilia Bussani, Mariarosaria Di Tommaso   +2 more
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Rapid diagnosis of trisomy 13 of maternal origin by quantitative fluorescent polymerase chain reaction analysis in a pregnancy with fetal holoprosencephaly, premaxillary agenesis, postaxial polydactyly of left hand and overriding aorta

Taiwanese Journal of Obstetrics & Gynecology, 2021
Objective: We present rapid diagnosis of trisomy 13 of maternal origin by quantitative fluorescent polymerase chain reaction (QF-PCR) in a pregnancy with multiple fetal abnormalities.
Chih-Ping Chen, Chao-Yun Wu, Schu-Rern Chern, Shin-Wen Chen, Fang-Tzu Wu, Mang-Shang Lee, Wayseen Wang   +6 more
doaj   +1 more source

Rapid Detection of Aneuploidies in Spontaneous Aborted Fetal Samples by Quantitative Fluorescence-PCR: A Descriptive Study [PDF]

Journal of Genetic Resources, 2021
Chromosomal aneuploidies are the most chromosomal abnormalities at birth due to maternal meiosis I errors. Pregnancies with autosomal chromosomal aneuploidies that survive are namely trisomies 13 (Patau syndrome), 18 (Edward syndrome), and 21 (Down ...
Hamid Reza Sharifzadeh, Majid Tafrihi, Nouredin Moradi, Naghmeh Gholipour   +3 more
doaj   +1 more source

Sequential application of copy number variation sequencing and quantitative fluorescence polymerase chain reaction in genetic analysis of miscarriage and stillbirth

Molecular Genetics & Genomic Medicine, 2023
Background Copy number variation sequencing (CNV‐seq) could detect most chromosomal abnormalities except polyploidy, and quantitative fluorescence polymerase chain reaction (QF‐PCR) is a supplementary method to CNV‐seq in triploid detection.
Quan Chen, Hao Zhang, Xue Li, Junxing Li, Huijuan Chen, Lin Liu, Shijie Zhou, Zhihong Xu   +7 more
doaj   +1 more source

Rapid confirmation of trisomy 13 of maternal origin by QF-PCR following postmortem tissue cell culture failure in a pregnancy with trisomy 13 at amniocentesis and fetal postaxial polydactyly and facial cleft

Taiwanese Journal of Obstetrics & Gynecology, 2022
Objective: We present rapid confirmation of trisomy 13 of maternal origin by quantitative fluorescent polymerase chain reaction (QF-PCR) following postmortem tissue cell culture failure in a pregnancy with trisomy 13 at amniocentesis and fetal postaxial ...
Chih-Ping Chen, Shin-Wen Chen, Jain-Pei Huang, Schu-Rern Chern, Fang-Tzu Wu, Yen-Ting Pan, Chen-Chi Lee, Wayseen Wang   +7 more
doaj   +1 more source

Application of immunofluorescence assay and nested polymerase chain reaction for query fever diagnosis in animal handlers of Puducherry, South India, and phylogenetic analysis based on IS1111 repetitive gene element [PDF]

Veterinary World, 2019
Background and Aim: Diagnosis of query fever (QF) is mostly done on the basis of serological/molecular tests, due to the stringent requirement of biosafety level-3 containment facilities for isolating Coxiella burnetii in culture.
Jothimani Pradeep, Selvaraj Stephen, Balakrishnan Sangeetha, Prabakar Xavier Antony, S. Amsaveni, Pratheesh Pooja   +5 more
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Rapid diagnosis of trisomy 18 of maternal origin by quantitative fluorescent polymerase chain reaction analysis following tissue culture failure for conventional cytogenetic analysis in a fetus with holoprosencephaly, ventricular septal defect, arthrogryposis of bilateral wrists and aplasia of the thumbs

Taiwanese Journal of Obstetrics & Gynecology, 2021
Objective: We present rapid diagnosis of trisomy 18 of maternal origin by quantitative fluorescent polymerase chain reaction (QF-PCR) analysis following tissue culture failure for conventional cytogenetic analysis in a fetus with holoprosencephaly (HPE),
Chih-Ping Chen, Shih-Shien Weng, Schu-Rern Chern, Shin-Wen Chen, Fang-Tzu Wu, Wayseen Wang   +5 more
doaj   +1 more source

Application of quantitative fluorescent polymerase chain reaction analysis for the rapid confirmation of trisomy 13 of maternal origin in a pregnancy with fetal holoprosencephaly, cyclopia, polydactyly, omphalocele and cell culture failure

Taiwanese Journal of Obstetrics & Gynecology, 2022
Objective: We present the application of quantitative fluorescent polymerase chain reaction (QF-PCR) for the rapid confirmation of trisomy 13 of maternal origin in a pregnancy with fetal holoprosencephaly (HPE), cyclopia, polydactyly, omphalocele and ...
Chih-Ping Chen, Liang-Kai Wang, Schu-Rern Chern, Shin-Wen Chen, Fang-Tzu Wu, Shin-Yin Huang, Wayseen Wang   +6 more
doaj   +1 more source

ANTENATAL DETECTION OF CHROMOSOMAL ABNORMALITIES COMBINING QF-PCR AND CYTOGENETIC ANALYSIS

Molecular and Experimental Biology in Medicine, 2020
Aim: To compare the diagnostic values and limitations of quantitative fluorescent polymerase chain reaction (QF-PCR) and conventional cytogenetic analysis in prenatal diagnosis of chromosomal abnormalities. Methods: A prospective study included simultaneous QF-PCR and cytogenetic analysis of 133 prenatal samples routinely obtained by amniocentesis or ...
Projić, Petar, Korać, Petra, Stipoljev, Feodora, Gjergja Juraški, Romana, Škaro, Vedrana, Mustapić, Ana   +5 more
openaire   +6 more sources