Results 321 to 330 of about 1,004,764 (341)

ddPCR surpasses classical qPCR technology in quantitating bacteria and fungi in the environment

Molecular Ecology Resources, 2022
Quantitative real‐time PCR (qPCR) has been widely used in quantifying bacterial and fungal populations in various ecosystems, as well as the fungi to bacteria ratio (F:B ratio).
Danrui Wang, Shang Wang, Xiongfeng Du, Qing He, Yuegao Liu, Zhujun Wang, Kai Feng, Yan Li, Ye Deng   +8 more
semanticscholar   +1 more source

Recommendations on qPCR/ddPCR Assay Validation by GCC.

Bioanalysis, 2022
Gene therapy, cell therapy and vaccine research have led to an increased use of qPCR/ddPCR in bioanalytical laboratories. CROs are progressively undertaking the development and validation of qPCR and ddPCR assays.
M. Wissel, Martin Poirier, C. Satterwhite, John H. Lin, Rafiq Islam, Jennifer S D Zimmer, Ardeshir Khadang, Jennifer Zemo, Todd Lester, M. Fjording, A. Hays, Nicola C Hughes, F. Garofolo, R. Guilbaud, E. Groeber, Heidi Renfrew, Kelly S. Colletti, Mathilde Yu, Jenny Lin, Xinping Fang, Santosh Shah, Wei Garofolo, S. Kar, R. Hayes, J. Pirro, Cheikh Kane, Marsha Luna, Allan Xu, Stephanie S Cape, M. O'Dell, Robert Wheller, H. Ritzen, J. Vance, Esme Farley, Katie Matys, E. Tabler, W. Mylott, Moucun Yuan, S. Karnik, T. Voelker, I. DuBey, C. Williard, Jing Shi, Jim Yamashita   +43 more
semanticscholar   +1 more source

MNAzyme qPCR: A Superior Tool for Multiplex qPCR

2013
Multicomponent nucleic acid enzymes (MNAzymes) are nucleic acid enzymes composed of multiple oligonucleotide partzymes that only associate to form catalytic complexes in the presence of a target nucleic acid. Once assembled, MNAzymes cleave a separate substrate (probe) between fluorophore and quencher labels to produce a fluorescent signal indicative ...
Elisa, Mokany, Alison V, Todd
openaire   +2 more sources

qPCR Genotyping of Polyploid Species

2023
A simple and cost-effective method for genotyping polyploid plants using quantitative PCR (qPCR) is described in this chapter. There is no additional operation, only simultaneous amplification of alleles and reference sequences with constant copy number in the genome.
Haiyan, Wang, Jiangbo, Dang, Qigao, Guo, Guolu, Liang   +3 more
openaire   +2 more sources

qPCR Detection of Candida auris Using the GPS™ CanAur MONODOSE dtec–qPCR Test

2022
Candida auris is a multidrug-resistant pathogenic ascomycete yeast of increasing health concern. C. auris colonizes patient's skin and can persist for weeks on surfaces, so it can be transmitted within and between hospitals. The most common diagnostic platforms in microbiology use reference databases that have not yet incorporated C.
Antonio, Martínez-Murcia, Gema, Bru, Aaron, Navarro   +2 more
openaire   +2 more sources