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Angewandte Chemie, 2018
The epitranscriptomic mark N6 -methyladenosine (m6 A) is the most abundant RNA modification in eukaryotic mRNA, but various limitations in currently available m6 A detection methods have precluded routine identification of m6 A marks at the single-site ...
Yu Xiao +5 more
semanticscholar +1 more source
The epitranscriptomic mark N6 -methyladenosine (m6 A) is the most abundant RNA modification in eukaryotic mRNA, but various limitations in currently available m6 A detection methods have precluded routine identification of m6 A marks at the single-site ...
Yu Xiao +5 more
semanticscholar +1 more source
Measuring RNAi Knockdown using qPCR
2013We describe a scalable method for measuring changes in gene expression following RNAi in mammalian cells. This protocol outlines methods to transfect cells, isolate total RNA, perform cDNA synthesis, run qPCR reactions with multiplexed TaqMan dual hydrolysis probes, and analyze the results from qPCR using relative quantification (Fleige et al., 2006 ...
Stuart, Milstein +3 more
openaire +2 more sources