Results 61 to 70 of about 2,097,297 (305)
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
A Method for Determining the Content of Glycoproteins in Biological Samples
Molecules, 2016 The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected ...
Yang Gao +5 more
doaj +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms [PDF]
, 2008 Background The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying genetically modified components in
Žel Jana +11 more
core +1 more source
Inferences in context : contextualism, inferentialism and the concept of universal quantification [PDF]
, 2009 This Thesis addresses issues that lie at the intersection of two broad philosophical projects: inferentialism and contextualism. It discusses and defends an account of the logical concepts based on the following two ideas: 1) that the logical concepts ...
Tabet, Chiara
core +2 more sources
Frontiers in Pharmacology, 2017 Solanum nigrum L., is traditionally used for the management of the various liver disorders. Investigating the effect of polarity based fractionation of S.
Karishma Chester +3 more
doaj +1 more source
Agronomy, 2022 Industrial hemp (Cannabis sativa L.) is a plant native to Asia, and is considered to be a primary source of food, textile fiber, and medicines. It is characterized by containing minimal concentrations of delta-9 tetrahydrocannabidol (THC), which is the ...
Carmen Jarén +5 more
doaj +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Metals, 2022 The long-term sustainability of the Additive Manufacturing (AM) industry not only depends on the ability to produce parts with reproducible quality and properties to a large extent but also on the standardization of the production processes.
Song Zhang +6 more
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source