Results 81 to 90 of about 228,684 (270)

Development of Diagnostic Tools for the Seed Potato Industry

, 2010
The Australian potato industry is threatened by inadequate measures to control the virus health of seed potatoes. Potatoes are vegetatively propagated; therefore infection can result in disease spreading through generations.
Mortimer-Jones, Sheila Mary
core  

Self‐Assembled Skin Equivalents with Monoclonal CRISPR/Cas9‐Modified N/TERT‐1 Keratinocytes: A Cutting‐Edge model for Human Skin and its Diseases

Advanced Healthcare Materials, EarlyView.
Self‐assembled, scaffold‐free full‐thickness skin equivalents with monoclonal, genetically modified N/TERT‐1 keratinocytes represent a novel in vitro model of human skin and skin diseases. The model is highly robust, reproducible, physiologically relevant, and suitable for high‐throughput applications.
Marta Slaufova, Tugay Karakaya, Michela Di Filippo, Thomas Kündig, Hans‐Dietmar Beer   +4 more
wiley   +1 more source

Formes oculaires de l'herpès virose féline [PDF]

, 2008
Le virus herpes de type 1 (FeHV-1), ou virus de la rhinotrachéite virale féline, est un agent infectieux spécifique des félidés. Caractérisé par sa forte virulence, son caractère contagieux ainsi que sa possibilité de récurrence, le virus FeHV-1 est très
Cohen, Stéphanie
core  

Adaptable Covalent Organic Framework‐Hydrogel Microneedles for Glucose‐Responsive Isoliquiritigenin Delivery in Diabetic Wound Therapy

Advanced Healthcare Materials, EarlyView.
We develop a covalent organic framework‐loaded Traditional Chinese Medicine monomer nanodrug delivery system (ISL@bCOF) via boron‐oxygen bonds, a smart response to glucose for delivering AI‐driven isoliquiritigenin (ISL) targeting ZBP1. This dynamic release mechanism, combined with transdermal delivery technology via potent microneedles, can modulate ...
Menghan Zhou, Zongjie Hu, Xiuwen Li, Huaman Geng, Yang Liu, Yajie Wang, Yunshu Yang, Guangdong Zhou, Yujie Hua, Di Wang   +9 more
wiley   +1 more source

ResonSense^\uae: simple linear fluorescent probes for quantitative homogeneous rapid polymerase chain reaction

Here we report a strand-specific fluorescent homogeneous assay format for rapid polymerase chain reaction (PCR). A number of similar assays are commonly used for research applications and are an ideal solution for a closed tube quantitative PCR.
Siddle AL, Page RH, Lee MA
core  

Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes

, 2014
Background: Real-time polymerase chain reaction (PCR) is based on the revolutionary method of PCR. This technique is the result of PCR enormous sensitivity and real-time monitoring combination.
Mohamadhasan Tajadini, Mojtaba Panjehpour, Shaghayegh Haghjooy Javanmard   +2 more
core   +1 more source

Viral Infection‐Inspired Autonomous Detection of Fusion‐Competent Viruses for Screening and Environmental Surveillance

Advanced Materials, EarlyView.
Inspired by viral entry mechanisms, the FUSION assay enables autonomous detection of respiratory viruses via membrane fusion–triggered CRISPR‐Cas13a activation. VEACON selectively fuses with fusion‐competent viruses, triggering fluorescence within confined vesicles.
Jae Chul Park, Yidam Song, Hyun‐Woo Choi, Jaeuk Sung, Harin Jin, WonSeok Gwak, Kirim Yoo, Soohwang Lee, Jongeon Park, Jeongmin Kim, Hye‐Jun Jo, Jahyun Koo, Youngdo Jeong, Kwan Hyi Lee, Seung‐Jung Kee, Hojun Kim   +15 more
wiley   +1 more source

Hierarchically Engineered Multi‐Enzyme Nanoreactors for in vitro Drug Biosynthesis and Pathway Transplantation Into Cells

Advanced Materials, EarlyView.
A hierarchically structured metal–organic framework (eMIL) is shown to host the complete six‐enzyme biosynthesis pathway for the anti‐cancer pigment violacein, reshaping reaction dynamics and multiplying in vitro yields. eMIL nanoreactors deliver this reconstituted bacterial multi‐protein system into mammalian cells, where cell‐derived substrates and ...
Ainur Sharip, Somayah S. Qutub, Manar M. Farooqui, Walaa Baslyman, Nida Khalfay, Lukman O. Alimi, Patricia Lopez Sanchez, Lingyun Zhao, Milena Chernyshevskaia, Giovanni Colombo, Niveen M. Khashab, Stefan T. Arold, Raik Grünberg   +12 more
wiley   +1 more source

Prenatal Invasive Diagnosis with Quantitative Fluorescent PCR

, 2011
Purpose: To develop the method of prenatal invasive diagnosis and compare it abilities with traditional genetic cytological examination.Materials and Methods: There was conducted prenatal fetal invasive diagnosis of 366 pregnant women.
V. V. Avrutskaja, S. Y. Kriger, R. A. Shokarev, V. S. Gimbut, N. V. Kriventsova   +4 more
core  

Quantification of poliovirus in seawater and sewage by competitive reverse transcriptase - polymerase chain reaction

, 1998
Reverse transcriptase - polymerase chain reaction (RT-PCR) has been used extensively to detect enteric viruses in environmental samples. Advantages of RT-PCR include its high detection sensitivity and rapid turn-around time.
Stephen L Parker, Yu-Li Tsai
core   +1 more source