Results 121 to 130 of about 698,289 (197)

Application of quantitative real-time PCR for identifying respiratory tract pathogens in outpatients with RTIs. [PDF]

Front Public Health
Qi L, Yang Y, Xu Z, Wang H, Pan Z, Zhao D, Liu R, Zhang H, Xie X.   +8 more
europepmc   +1 more source

Aqueous Two‐Phase Bioinks for Discrete Packing and Compartmentalization of 3D Bioprinted Cells

Advanced Healthcare Materials, EarlyView.
Aqueous two‐phase systems (ATPS) enable the formation of biomimetic interfaces crucial for tissue engineering. However, clinical translation remains limited by the challenge of precisely controlling cellular compartmentalization. Here, we developed ATPS biomaterial inks for 3D bioprinting allowing tuneable droplet formation via NaCl modulation.
Martina Marcotulli, Arianna Iacomino, Federico Serpe, Lucia Iafrate, Marco Bastioli, Giorgia Montalbano, Biagio Palmisano, Silvia Franco, Roberta Angelini, Alessandro Corsi, Mara Riminucci, Giancarlo Ruocco, Chiara Scognamiglio, Andrea Barbetta, Gianluca Cidonio   +14 more
wiley   +1 more source

Selection of <i>Bactrocera tau</i> (Walker) Reference Genes for Quantitative Real-Time PCR. [PDF]

Insects
Zhai Y, Yu Y, Xu P, Zeng X, Long X, Wei D, He Z, Gao X.   +7 more
europepmc   +1 more source

Preliminary Study of a Ninj1‐Loaded Bimodal Ultrasound/NIR Fluorescence Targeted Molecular Probe for Diagnosing Early‐Stage Inflammation in Coronary Microvascular Dysfunction

Advanced Healthcare Materials, EarlyView.
Coronary microvascular dysfunction (CMD) targeting remains a challenge for precise diagnosis. This work presents a dual‐modal nanoprobe (T‐IR780‐NBs) that combines ultrasound contrast with near‐infrared fluorescence. This technology utilizes proteomics‐derived antibodies that specifically localize to inflamed and injured cardiac tissue, enabling ...
Xiaohui Xu, Lina Guan, Baihetiya Tayier, Chen Yuan, Shangke Chen, Qi Shi, Yuming Mu   +6 more
wiley   +1 more source

Selection of <i>Sclerodermus pupariae</i> Reference Genes for Quantitative Real-Time PCR. [PDF]

Insects
Zhou T, Feng H, Zhang J, Tang Y, Dong X, Kang K.   +5 more
europepmc   +1 more source

A 3D Bioprinted Spheroid‐Laden dECM‐Enriched Osteosarcoma Model for Enhanced Drug Testing and Therapeutic Discovery

Advanced Healthcare Materials, EarlyView.
A 3D biomimetic OS model was developed by bioprinting an OS‐cell‐derived dECM‐enriched bioink with OS spheroids incorporated. The model showed upregulation of known OS prognostic markers and increased resistance to doxorubicin, compared to 2D cultures and scaffold‐free spheroids, making this a more clinically relevant platform for drug discovery ...
Margarida F. Domingues, Maria Catarina Carreira, Mafalda S. Santos, Daniela Pacheco, Frederico Castelo Ferreira, Paola Sanjuan‐Alberte, João Carlos Silva   +6 more
wiley   +1 more source

The Role of Quantitative Real-Time PCR in the Invasive Pulmonary Aspergillosis Diagnosis: A Retrospective Study. [PDF]

Microorganisms
Trovato L, Calvo M, Palermo CI, Scalia G.   +3 more
europepmc   +1 more source

Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in the Bean Bug, Riptortus pedestris (Hemiptera: Alydidae). [PDF]

Insects, 2023
Wang L, Liu Q, Guo P, Gao Z, Chen D, Zhang T, Ning J.   +6 more
europepmc   +1 more source

SiO2‐CaOCME/Poly(Tetrahydrofuran)/Poly(Caprolactone) 3D‐Printed Scaffolds Drive Human‐Bone Marrow Stromal Cell Osteogenic Differentiation

Advanced Healthcare Materials, EarlyView.
3D printed hybrid scaffolds combining bioactive silica–calcium chemistry with elastic polymers guide human bone stem cells to form bone. The scaffolds support cell survival, organization, and invasion while releasing osteogenic ions. Together, architecture and composition drive bone‐specific gene expression, extracellular matrix organization, and ...
David R. Sory, Agathe C.M. Heyraud, Julian R. Jones, Sara M. Rankin   +3 more
wiley   +1 more source

Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2. [PDF]

Pathogens
Giovambattista G, Bao A, Marcuzzi O, Vega AL, Rico JAP, Ortega Masague MF, Rojas LAC, Martinez RD, Reinosa OU, Aida Y.   +9 more
europepmc   +1 more source