Results 1 to 10 of about 84 (70)
The Kiss Switch Brings Inactive R3C Ligase Ribozyme Back to Life [PDF]
Biology, 2018 R3C ligase ribozyme catalyzes the nucleophilic attack by a 3′-hydroxyl on a 5′-α-phosphorus of triphosphates to form a 3′-5′-phosphodiester bond. In the present study, although the truncation of R3C ribozyme was accompanied by a large reduction in ...
Kana Tanizawa +4 more
doaj +7 more sources
Development of a Functionally Minimized Mutant of the R3C Ligase Ribozyme Offers Insight into the Plausibility of the RNA World Hypothesis [PDF]
Biology, 2014 The R3C ligase ribozyme is an artificial ligase ribozyme produced by modification of the ribozyme that lacks cytidine. Here, we attempted to modify the original R3C ribozyme (73 nucleotides) by reducing the number of nucleotides while maintaining the ...
Eri Kurihara +3 more
doaj +7 more sources
Development of Allosteric Ribozymes for ATP and l-Histidine Based on the R3C Ligase Ribozyme [PDF]
LifeDuring the evolution of the RNA, short RNAs are thought to have joined together to form long RNAs, enhancing their function as ribozymes. Previously, the artificial R3C ligase ribozyme (73 nucleotides) was successfully reduced to 46 nucleotides; however,
Yuna Akatsu +2 more
doaj +6 more sources
Acquisition of Dual Ribozyme-Functions in Nonfunctional Short Hairpin RNAs through Kissing-Loop Interactions [PDF]
Life, 2022 The acquisition of functions via the elongation of nucleotides is an important factor in the development of the RNA world. In our previous study, we found that the introduction of complementary seven-membered kissing loops into inactive R3C ligase ...
Hiromi Mutsuro-Aoki, Koji Tamura
doaj +2 more sources
Effects of lipid membranes on RNA catalytic activity and stability. [PDF]
Biol CellThis study explores how lipid membranes influence the activity and stability of natural ribozymes. We found that lipid membranes can both decrease and enhance ribozyme activity. We also observed that lipid membranes promote RNA degradation, depending on lipid composition and membrane fluidity.
Czerniak T, Saenz JP.
europepmc +2 more sources
Engineering of Small Ribozymes Acting on RNA: What is Needed to Make a New Function Work with an Existing Catalyst? [PDF]
ChembiochemRational design is a potent strategy for developing ribozymes with advanced functionalities. Engineered variants of the hairpin ribozyme impressively demonstrate how function can be tuned through structural manipulation. The engineering of nucleic acids has been a longstanding objective in research, with the field gaining significant attention ...
Ebermann C, Müller S.
europepmc +2 more sources
Charge-density reduction promotes ribozyme activity in RNA–peptide coacervates via RNA fluidization and magnesium partitioning [PDF]
Nature Chemistry, 2022 Juan M Iglesias-Artola +2 more
exaly +2 more sources
Angewandte Chemie, Volume 135, Issue 17, April 17, 2023., 2023 Temperatursensitive RNA‐Organellen wurden in Riesenvesikeln verkapselt und dazu genutzt, die Reaktionsraten eines minimalen katalytischen RNA Netzwerkes zu erhöhen. Durch anschließendes Einfrieren und Auftauen wurde beabsichtigt, Vesikelmaterial zu vermischen und somit eine durch Organellen begünstigte Propagation solcher katalytischen Netzwerke ...
Benedikt Peter +2 more
wiley +1 more source
Angewandte Chemie International Edition, Volume 62, Issue 17, April 17, 2023., 2023 Temperature‐responsive RNA organelles inside giant unilamellar vesicles were exploited to increase catalytic rates of a minimal catalytic RNA network. Subsequent cycles of freezing and thawing serve as physico‐chemical driver to exchange vesicle contents aiming at an efficient organelle‐aided propagation of such catalytic networks across lipid vesicles.
Benedikt Peter +2 more
wiley +1 more source
Abiogenesis through gradual evolution of autocatalysis into template‐based replication
FEBS Letters, Volume 597, Issue 3, Page 344-379, February 2023., 2023 In this review, we examine how rudimentary forms of evolution may have started in autocatalytic RNA networks and subsequently led to the appearance of RNA replicases, allowing life to emerge from inanimate matter. How life emerged from inanimate matter is one of the most intriguing questions posed to modern science.
Polina Pavlinova +3 more
wiley +1 more source