Results 1 to 10 of about 5,224 (170)

Investigation of acquired dysfibrinogenaemia in adult patients with sepsis using fibrinogen function vs. concentration ratios: a cross-sectional study [PDF]

Frontiers in Medicine, 2023
IntroductionInherited or acquired molecular abnormalities form a clinically heterogeneous group of fibrinogen disorders called dysfibrinogenaemia.
Rosa Toenges, Michael Steiner, Christian Friedrich Weber, Christian Friedrich Weber, Wolfgang Miesbach   +4 more
doaj   +2 more sources

Lactoferrin concentration in bovine milk: validation of radial immunodiffusion technique, sources of variation, and association to udder health status

Italian Journal of Animal Science, 2023
The present study aimed to evaluate analytical performances of radial immunodiffusion (RID) technique for the quantification of milk lactoferrin (LF), and to investigate the main sources of variation of LF concentration in individual milk samples of ...
Massimo De Marchi, Mauro Penasa, Giovanni Niero   +2 more
exaly   +2 more sources

Effect of High-Pressure Processing Operating Parameters on Microbial Inactivation and Bioactive Protein Preservation in Bovine Milk: A Systematic Review. [PDF]

Compr Rev Food Sci Food Saf
ABSTRACT In the U.S., bovine milk is processed using thermal pasteurization to ensure microbial safety. However, this process alters the structure of heat‐sensitive bioactive proteins associated with the functional benefits of raw milk, including antimicrobial, immunomodulatory, and antioxidant proteins.
Sykora R, Mark C, Biondi Ryan M, Barman B, Pitino M, Dallas DC.   +5 more
europepmc   +2 more sources

Comparison of radial immunodiffusion, turbidimetric immunoassay, and Brix refractometry for determining bovine colostrum quality [PDF]

JDS Communications
Determining the concentration of IgG in colostrum is critical for assessment of colostrum quality. On-farm use of a Brix refractometer to estimate colostrum IgG concentration is widespread, whereas radial immunodiffusion (RID) is the laboratory reference
T.A. Westhoff, E.L. Behling-Kelly, S. Mann   +2 more
doaj   +2 more sources

Diagnostic accuracy of a Brix refractometer to estimate porcine colostrum quality. [PDF]

Vet Rec
Abstract Background The objective of this study was to investigate the validity of the Brix refractometer to accurately estimate immunoglobulin G (IgG) concentrations in porcine colostrum, using radial immunodiffusion (RID) as a reference standard. Methods Eighty‐seven composite colostrum samples were collected from sows on a single pig farm between ...
O'Brien ÁC, Farish M, Baxter EM, Denholm KS.   +3 more
europepmc   +2 more sources

A Monoclonal Antibody ELISA-Based Assay for Measuring the Potency of Candidate H5 Clade 2.3.4.4b Pandemic Influenza Vaccines. [PDF]

Influenza Other Respir Viruses
ABSTRACT Background The single radial immunodiffusion (SRID) assay is used to determine the potency of inactivated influenza vaccines. Nevertheless, development of alternative influenza vaccine potency assays with greater sensitivity and less reliance on large quantities of reference reagents is needed.
Odin M, Schmeisser F, Soto J, Weir JP.
europepmc   +2 more sources

Severe Pulmonary Coccidioidomycosis Treated With Systemic Corticosteroids in an Adolescent Child. [PDF]

Case Rep Pediatr
Coccidioidomycosis (CM) is an emerging public health challenge due to an increasing number of cases in the U.S. Diagnosis may be challenging because of its nonspecific presentation, which may lead to treatment delays. In addition, the optimal management of severe pulmonary CM remains unclear.
Shinozaki R, Krogstad P, Adachi K, Bulut Y.   +3 more
europepmc   +2 more sources

Investigation of brix refractometry for estimating bovine colostrum immunoglobulin G concentration [PDF]

Frontiers in Veterinary Science, 2023
Many dairy operations uses a Brix refractometer to assess the quality of first-milking colostrum. This study investigated whether a digital Brix refractometer could be used in a model to predict colostrum IgG concentration and whether more than one %Brix
Donald Sockett, Ryan M. Breuer, Ryan M. Breuer, Lindsey W. Smith, Nicholas S. Keuler, Thomas Earleywine   +5 more
doaj   +2 more sources

Developing tools for learning immunology using diffusion-based salt precipitation assays: A low-cost alternative for college laboratories. [PDF]

Biochem Mol Biol Educ
Abstract The Ouchterlony double immunodiffusion technique is used as a teaching tool for studying immune responses and exemplifying differences in antigen–antibody reactions. Although commonplace in undergraduate labs, standardized commercial kits limit learning experiences because they have fixed modalities of use, a low shelf‐life, and impose ...
Pal A, Sen S.
europepmc   +2 more sources

Determination of Fibrinogen Ratio Cutoff Limits Using Indirect Reference Interval Methodology. [PDF]

Int J Lab Hematol
ABSTRACT Introduction Discordant fibrinogen antigen to activity ratios are utilized by clinicians as evidence of dysfibrinogenemia. Abnormal ratio cutoffs implemented by clinical laboratories are typically determined by validation studies that include limited numbers of samples.
Saadalla A, Doyle K, Moser K, Smock K.
europepmc   +2 more sources