Results 161 to 170 of about 5,650 (193)

Radial immunodiffusion assay for rat α1-acid glycoprotein

Pharmacology Biochemistry and Behavior, 1990
alpha 1-Acid glycoprotein (AGP) is an "acute phase protein" whose expression is altered in several human pathologies. Using antiserum from New Zealand white rabbits, a radial immunodiffusion assay for measuring AGP levels in rat plasma was developed operating in the range of 50-2500 micrograms/ml with high specificity. Standard curves were constructed (
F J, Arnold, L R, Meyerson
openaire   +2 more sources

A Staining Rack for Radial Immunodiffusion Plates

American Journal of Clinical Pathology, 1970
A readily constructed rack is described tha permits the uniform handling of as many as 10 radial immunodiffusion plates simultaneously during antibody elution and subsequent staining. The device consists of a circular baseplate with a centrally located, vertical axial rod and a series of hollow cylindrical spacers that thread onto the rod.
openaire   +2 more sources

Quantitative Radial Immunodiffusion

1979
Mancini et al. (1965) developed a very simple method to quantitate antigens using gel diffusion. The principle is this: The antigen is placed in a well punched into an agar layer. The agar was mixed while melted at 45°-50°C with the corresponding antibody.
openaire   +1 more source

Single Radial Immunodiffusion

1996
Single radial immunodiffusion is used extensively for the quantitative estimation of antigens (1). In this method, the antigen--antibody precipitation is made more sensitive than in double immunodiffusion (see Chapter 135) by the incorporation of the antiserum in the agar solution before the gel is made (2). Thus, the antiserum is uniformly distributed
openaire   +1 more source

Quantitative radial immunodiffusion assay for serum amyloid A protein

Journal of Immunological Methods, 1983
A radial immunodiffusion assay for serum amyloid A protein (SAA) using a commercially available antiserum is described. Serum is applied untreated to 1% agarose gels prepared in 0.02 M barbitone buffer, pH 8.6, containing 40 g/l polyethylene glycol 6000. Incubation is carried out overnight at 37 degrees C.
R E, Chambers, J T, Whicher
openaire   +2 more sources

[20] Single radial immunodiffusion

1981
Publisher Summary This chapter describes the different aspects of single radial immunodiffusion (SRID). SRID is an immunodiffusion technique, in which a single partner of the antigen–antibody reaction, usually the antigen (Ag), diffuses radially from a small well punched into a gel layer of constant thickness and the other partner, usually the ...
openaire   +1 more source

Improvements to the enzyme-developed radial immunodiffusion technique

Journal of Immunological Methods, 2002
An enzyme-developed radial immunodiffusion technique, previously known as the diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA), has been improved in two ways: (a) antibody-containing spots have been made larger and more distinct by revealing them with a mixture of hydrogen peroxide, 3,3'-diaminobenzidine and nickel, and further ...
openaire   +2 more sources

Radial immunodiffusion and rocket Immunoelectrophoresis

1979
Specific protein concentrations may be measured in gel media by two techniques: single radial immunodiffusion and rocket Immunoelectrophoresis. The former method is called ‘single’ to distinguish it from the qualitative Ouchter-lony or ‘double diffusion’ methods, and ‘radial’ to contrast it with the earlier ‘linear diffusion’ technique of Oudin1 ...
openaire   +1 more source

Determination of relative antigen-antibody avidities by radial immunodiffusion

Journal of Immunological Methods, 1983
Radial immunodiffusion can be used to determine relative antigen-antibody avidities in exactly the same way as demonstrated previously for quantitative immunoelectrophoresis (Birkmeyer et al., 1981). Antigen-antibody interactions of greater avidity result in a greater value of (delta Area/delta [Antigen]) in plots of immunoprecipitin circle area versus
A L, Tan-Wilson, B R, Rightmire, K A, Wilson   +2 more
openaire   +2 more sources

DETERMINATION OF ANTISTREPTOLYSIN O BY REVERSED SINGLE RADIAL IMMUNODIFFUSION

Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology, 1974
A diffusion‐in‐gel micromethod for quantitation of ASO is presented. The method is compared with conventional dilution techniques. Non‐reduced ASO and sheep erythrocytes were mixed in an agarose gel. Around holes filled with sera under test zones of inhibited hemolysis appeared when the ASO was reduced.
openaire   +2 more sources