Results 171 to 180 of about 61,308 (224)
Some of the next articles are maybe not open access.
A radioimmunoassay for progesterone
Steroids, 1972Abstract The need for a simple and fast quantitative method for progesterone with routine laboratory and clinical application motivated the development of a radioimmunoassay. Progesterone antibodies were produced in rabbits by immunization with a bovine serum albumin conjugate of 11α-hydroxyprogesterone hemisuccinate.
J M, Spieler +3 more
openaire +2 more sources
A radioimmunoassay of androstenedione
Steroids, 1973Abstract A dextran-coated charcoal radioimmunoassay for androstenedione (4-androsten-3, 17-dione) is reported which uses an anti-testosterone antiserum raised in sheep, against a testosterone-17-hemisuccinate-Bovine Serum Albumin conjugate. It is more sensitive and rapid than previously published double dilution, gas chromatographic and competitive ...
I H, Thorneycroft +3 more
openaire +2 more sources
Science, 1970
The development of a radioimmunoassay for morphine is described. The hapten morphine is made antigenic by coupling it to a protein at the phenolic group of the molecule. Extremely low concentrations of morphine (0.5 nanogram) can be measured by this assay procedure.
S, Spector, C W, Parker
openaire +2 more sources
The development of a radioimmunoassay for morphine is described. The hapten morphine is made antigenic by coupling it to a protein at the phenolic group of the molecule. Extremely low concentrations of morphine (0.5 nanogram) can be measured by this assay procedure.
S, Spector, C W, Parker
openaire +2 more sources
The Journal of Clinical Endocrinology & Metabolism, 1975
A radioimmunoassay for melatonin has been developed after the raising of anti-melatonin antibodies in rabbits.
J, Arendt, L, Paunier, P C, Sizonenko
openaire +2 more sources
A radioimmunoassay for melatonin has been developed after the raising of anti-melatonin antibodies in rabbits.
J, Arendt, L, Paunier, P C, Sizonenko
openaire +2 more sources
A radioimmunoassay for diphenylhydantoin
Clinica Chimica Acta, 1973Abstract A radioimmunoassay for the anticonvulsant compound diphenylhydantoin (DPH) has been developed. Antisera were prepared by immunizing animals with a DPH-chicken γ-globulin conjugate. The assay depends on the capacity of unlabelled DPH to compete with a known amount of [14C]DPH for the antibody-binding sites. The assay can detect as little as 0.
R E, Tigelaar +3 more
openaire +2 more sources
Angewandte Chemie International Edition in English, 1976
AbstractBiologically active substances are often effective in nanogram or even smaller quantities. Investigations of their mode of action therefore require highly sensitive microanalytical methods of detection. Chemical, chromatographic or spectrometric methods frequently lack the required sensitivity; it was only after the discovery of the ...
openaire +2 more sources
AbstractBiologically active substances are often effective in nanogram or even smaller quantities. Investigations of their mode of action therefore require highly sensitive microanalytical methods of detection. Chemical, chromatographic or spectrometric methods frequently lack the required sensitivity; it was only after the discovery of the ...
openaire +2 more sources
Gastroenterologia Japonica, 1979
A sensitive and specific radioimmunoassay for rat and human serum ligandin was developed using antisera generated in rabbits by the injection of ligandin purified from liver. Because rat ligandin and human ligandin have no cross reactivity, radioimmunoassay systems for each of the serum ligandins were prepared. Highly purified ligandin labeled with125I
Masanobu Tsuru, S. Murao
openaire +2 more sources
A sensitive and specific radioimmunoassay for rat and human serum ligandin was developed using antisera generated in rabbits by the injection of ligandin purified from liver. Because rat ligandin and human ligandin have no cross reactivity, radioimmunoassay systems for each of the serum ligandins were prepared. Highly purified ligandin labeled with125I
Masanobu Tsuru, S. Murao
openaire +2 more sources
2003
Radioimmunoassay is often described in terms of the competition between a radiolabeled antigen (Ag*) and its unlabeled counterpart (Ag) for binding to a limited amount of specific antibody (Ab) (1). In most radioimmunoassays the reaction is allowed to proceed to equilibrium and thus, can be represented by Eq. (1). [Formula: see text].
openaire +4 more sources
Radioimmunoassay is often described in terms of the competition between a radiolabeled antigen (Ag*) and its unlabeled counterpart (Ag) for binding to a limited amount of specific antibody (Ab) (1). In most radioimmunoassays the reaction is allowed to proceed to equilibrium and thus, can be represented by Eq. (1). [Formula: see text].
openaire +4 more sources
Radioimmunoassay for immunoconglutinins
Journal of Immunological Methods, 1980A competitive radioimmunoassay for immunoconglutinin (IK) was developed using isolated antostimulated rabbit IK labelled with 125I and glutaraldehyde-fixed sheep erythrocytes coated with fixed complement (EAC). IKs in test sera of human or animal origin produced specific inhibition of uptake of [125I]IK onto EAC and this was quantitated by reference to
M, Druguet +3 more
openaire +2 more sources
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1982
We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 micrograms/ml (15 microM) digitonin. The assay is
G, Brooker, J W, Fleming, E M, Ross
openaire +2 more sources
We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 micrograms/ml (15 microM) digitonin. The assay is
G, Brooker, J W, Fleming, E M, Ross
openaire +2 more sources