Results 181 to 190 of about 61,308 (224)
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Radioimmunoassay of Angiotensin
Nature, 1968HABER et al.1 showed that antibodies elicited in rabbits by copolymers of angiotensin with poly-L-lysine can be used for radioimmunoassay of angiotensin. The following are details and some results of the method we have developed. Comparing the binding affinity of three different rabbit antisera to Val5-angiotensin II amide (‘Hypertensin’, Ciba) and ...
H J, Hollemans +2 more
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Seminars in Nuclear Medicine, 1975
The powerful nuclear medicine tool of radioimmunoassay was very rapidly developed because of the favorable properties of insulin in such a procedure. The current method of insulin immunoassay in our laboratory is described, with emphasis on the details of the separation of bound and free using talc tablets; emphasis is also given to the correction for ...
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The powerful nuclear medicine tool of radioimmunoassay was very rapidly developed because of the favorable properties of insulin in such a procedure. The current method of insulin immunoassay in our laboratory is described, with emphasis on the details of the separation of bound and free using talc tablets; emphasis is also given to the correction for ...
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Biochimica et Biophysica Acta (BBA) - Protein Structure, 1977
Abstract A sensitive and specific radioimmunoassay has been developed for rat ligandin, capable of measuring this protein in the manogram range. The assay employs [125I] ligandin prepared by the chloramine-T method and rabbit antiserum to rat ligandin, with a double antibody method of separating antibody-bound from free ligandin.
N M, Bass +3 more
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Abstract A sensitive and specific radioimmunoassay has been developed for rat ligandin, capable of measuring this protein in the manogram range. The assay employs [125I] ligandin prepared by the chloramine-T method and rabbit antiserum to rat ligandin, with a double antibody method of separating antibody-bound from free ligandin.
N M, Bass +3 more
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Radioimmunoassay of the A prostaglandins
Prostaglandins, 1972Antibodies to the A prostaglandins (PGA) were produced in rabbits immunized with a conjugate of prostaglandin E2 (PGE2) covalently linked to bovine serum albumin (BSA) by reaction with carbodiimide reagent. A radioimmunoassay was developed using dextran-coated charcoal to separate the free from antibody bound PGA1-3H.
R M, Zusman +3 more
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Radioimmunoassay for rhodopsin
Experimental Eye Research, 1980Abstract A radioimmunoassay for rhodopsin was devised using [125I]rhodopsin and antibody to purified bovine rhodopsin in a double antibody procedure that can quantify the concentration of this visual pigment when present in picomolar amounts. The method demonstrated a high degree of sensitivity, reproducibility, and specificity, and is applicable to ...
B B, Lentrichia +2 more
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1978
Several sensitive radioimmunoassay systems for secretin have been developed and reported1–6. The development of these assay systems depended upon the generation of a specific secretin antiserum and the iodination of secretin (natural porcine secretin, synthetic secretin or a tyrosyl derivative of secretin).
P L, Rayford +2 more
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Several sensitive radioimmunoassay systems for secretin have been developed and reported1–6. The development of these assay systems depended upon the generation of a specific secretin antiserum and the iodination of secretin (natural porcine secretin, synthetic secretin or a tyrosyl derivative of secretin).
P L, Rayford +2 more
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Science, 1973
The development of a radioimmunoassay for serotonin is described. Serotonin is made antigenic by coupling it to bovine serum albumin. Thrombocyte-free plasma instead of serum from the immunized animals is used for radioimmunoassay. Less than 1 ng serotonin can be measured by this procedure.
B, Peskar, S, Spector
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The development of a radioimmunoassay for serotonin is described. Serotonin is made antigenic by coupling it to bovine serum albumin. Thrombocyte-free plasma instead of serum from the immunized animals is used for radioimmunoassay. Less than 1 ng serotonin can be measured by this procedure.
B, Peskar, S, Spector
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Radioimmunoassay of polyacrylamide
Experientia, 1978Antiserum to polyacrylamide has been produced in rabbits. It can be used in a sensitive radioimmunoassay for quantitation of polyacrylamide at very low concentrations. This novel approach to the analysis of a synthetic, biologically inactive, hydrocarbon polymer has potentially widespread implications, for example, in the downstream measurement of ...
P A, Drewes, A O, Kamp, J W, Winkelman
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The radioimmunoassay of buprenorphine
European Journal of Clinical Pharmacology, 1980Antisera to buprenorphine were obtained in rabbits immunised with 3-0-carboxymethylbuprenorphine and N-hemisuccinyl-norbuprenorphine conjugated to bovine serum albumin. Using the latter antiserum and tritium labelled buprenorphine a radioimmunoassay have good accuracy and precision was developed for concentrations as low as 50 picograms in 1 ml of ...
A J, Bartlett +6 more
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A RADIOIMMUNOASSAY OF THYROXINE
The Journal of Clinical Endocrinology & Metabolism, 1971ABSTRACT A simple, sensitive, specific and precise radioimmunoassay (RIA) for measurement of serum thyroxine (T4) is described. The RIA employs rabbit anti-thyroglobulin antiserum as the T4-binding protein. The precision, reproducibility and practicality of the RIA are comparable to those of competitive protein binding assay (CPBA) using serum TBG as ...
I J, Chopra, D H, Solomon, R S, Ho
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