Results 151 to 160 of about 822,055 (188)

Optimization of Radioligand Binding and Radioimmuno Assays

Analytical Biochemistry, 1996
Radioligand binding assay (RLA) and radioimmunoassay (RIA) development involves consideration of a large number variables which influence signal readout or system errors, with contrary effects on assay performance. We have developed an optimization strategy according to statistical criteria which incorporates these variables and which specifies a ...
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GPCR-radioligand binding assays

2016
Radioligand binding assays provide sensitive and quantitative information about guanine nucleotide protein G protein-coupled receptor (GPCR) expression and affinity for a wide variety of ligands, making them essential for drug structure-activity studies and basic GPCR research.
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Radioligand Binding Assays

1984
Early work aimed at examining the properties of chemical transmission focused on presynaptic events such as neurotransmitter synthesis, degradation, accumulation and release. In contrast, because of technical limitations, less information was obtained in regard to the biochemical events associated with neurotransmitter receptors.
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Radioligand Binding Assays: Theory and Practice

1996
Transmitters and hormones generate signals in target cells by interacting with a signal transducing protein or receptor. Receptors may be located in the cytosol, allowing for example, steroid and thyroid hormones to control gene transcription. However, the largest family comprises G-protein linked seven transmembrane spanning receptors located in the ...
Anthony P. Davenport, Fraser D. Russell
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Lecture II: Radioligand Assays

1977
Many assay methods have been developed in which antigenicity is estimated with the aid of radioisotopes. They require very small quantities of preparations. In radioimmunoassays antigens are labelled with radioisotopes, whereas in immunoradiometric assays antibodies are labelled; from the statistical viewpoint, the two types are similar. The literature
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A Sensitive Radioligand Binding Assay for Timolol in Plasma

Journal of Pharmaceutical Sciences, 1991
A sensitive and reproducible radioreceptor assay (RRA) is described for the determination of low picogram levels of timolol in plasma. The plasma or serum samples (1 mL) are prepared by selectively extracting timolol with lipophilic solvents or, alternatively, only plasma (serum) proteins are precipitated prior to binding assay. The recovery of timolol
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Radioligand-binding assays for study of neurotensin receptors

1989
Publisher Summary This chapter discusses the radioligand-binding assays for the study of neurotensin receptors. Radioligand-binding studies have provided valuable information concerning the characteristics, the structure–activity relationships, and the cellular localization of neurotensin receptors.
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BIOLOGICAL RECEPTORS: IMPLICATIONS OF RADIOLIGAND QUALITY IN BINDING ASSAYS

Journal of Biological Systems, 2002
We tested the ability of different fitting procedures to discern differences in the binding affinity between the labeled and unlabeled ligand in a receptor assay, in order to obtain the correct value of the radiotracer specific activity. This point is critical for the correct conversion of the estimated binding constants to meaningful units.
Venturino, Andrés, Rivera, Elena S.
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A Method for Radioligand Binding Assays Using a Robotic Workstation

Journal of Receptor Research, 1993
Radioligand binding assays provide a powerful tool for screening drug candidates at many receptors. We present a method utilizing a robotic workstation, the Biomek 1000, which automates the tedious and repetitive tasks of these assays. First, the robot handles the serial dilution of up to 8 drugs with 11 concentrations per drug. The sequential addition
B, Cusack, E, Richelson
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Radioligand Binding Assays for Adenosine Receptors

1990
The technique of radioligand binding is one that has been of considerable importance in defining receptor function at the molecular level. In addition to providing evidence for the potential existence of receptor subtypes, subclasses, and states (Williams and Enna, 1986; Williams and Sills, 1989), the ease and resource efficiency of the technique makes
Michael Williams, Kenneth A. Jacobson
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