Results 11 to 20 of about 2,283,209 (217)
Synthesis of Sulochrin-125I and Its Binding Affinity as α-Glucosidase Inhibitor using Radioligand Binding Assay (RBA) Method [PDF]
Atom Indonesia, 2014 Most of diabetics patients have type 2 diabetes mellitus or non insulin dependent diabetes mellitus. Treatment type 2 diabetes mellitus can be done by inhibiting α-glucosidase enzyme which converts carbohydrates into glucose.
W. Lestari +7 more
doaj +6 more sources
Pteridines, 1989 An enzyme-linked immunosorbent assay (ELISA) was calibrated and used for quantitation of the folate binding protein in human cerebrospinal fluid. The precision of the assay in terms of the coefficient of variation was 6.3% intra-assay (10 replicate ...
Ingemann Hansen Steen +2 more
doaj +2 more sources
Journal of Pharmacological Sciences, 2005 The radioligand binding assay technique is an extremely powerful tool for studying receptors. It allows an analysis of the interactions of hormones, neurotransmitters, and related drugs with their receptors.
Ikunobu Muramatsu +8 more
doaj +1 more source
Sodium Ions Affect GPR17 Conformational States and Functionality. [PDF]
ProteinsABSTRACT Sodium is an allosteric modulator of several class‐A GPCR regulating active versus inactive conformation state and may be a critical issue for the optimization of crystallization procedures. Herein, the role of Na+ in the regulation of GPR17 conformational states was studied through MD simulations of GPR17 wild‐type and mutated on two ...
Palazzolo L +6 more
europepmc +2 more sources
A Radioligand Binding Assay for Antitubulin Activity in Tumor Cells [PDF]
SLAS Discovery, 2006 The benzamide RH-5854 is shown to be highly potent toward tumor cells and to arrest nuclear division by a highly specific covalent binding to the beta-subunit of tubulin in the colchicine binding region. Binding of 3H-RH-5854 to beta-tubulin in HCT-116 colon cancer cells is saturable and has been exploited in the development of a cell-based competitive
David H, Young +2 more
openaire +2 more sources
[3H]-Spiperone Saturation Binding to Dopamine D2, D3 and D4 Receptors
Bio-Protocol, 2013 This protocol is intended for use in 96 well plates (1,200 μl wells) but it can similarly be applied to standard test tubes (Levant, 2007). D2, D3, and D4 dopamine receptors are members of the D2-like class of dopamine receptors.
Jan-Peter Wieringen, Martin Michel
doaj +1 more source
Validation of a [3H]Astemizole Binding Assay in HEK293 Cells Expressing HERG K+ Channels
Journal of Pharmacological Sciences, 2004 A radioligand binding assay for the HERG (human ether-a-go-go-related gene) K+ channel was developed to identify compounds which may have inhibitory activity and potential cardiotoxicity.
Peter J.S. Chiu +8 more
doaj +1 more source
A partially automated radioligand binding assay system for use in clinical and pharmaceutical research [PDF]
, 1993 Using a Tecan robotic sample processor and IBM compatible PCs we have developed a flexible, partially automated radioligand binding assay system. It handles pipetting parameters of up to 16 saturation or competition experiments at a time with up to 24 ...
Liebl, B. +4 more
core +1 more source
[3H]-Spiperone Competition Binding to Dopamine D2, D3 and D4 Receptors
Bio-Protocol, 2013 This protocol is intended for use in 96 well plates (1,200 μl wells) but it can similarly be applied to standard test tubes (Levant, 2007). D2, D3, and D4 dopamine receptors are members of the D2-like class of dopamine receptors.
Jan-Peter Wieringen, Martin Michel
doaj +1 more source
PLoS Computational Biology, 2021 Adenosine receptors (ARs) have been demonstrated to be potential therapeutic targets against Parkinson's disease (PD). In the present study, we describe a multistage virtual screening approach that identifies dual adenosine A1 and A2A receptor ...
Mukuo Wang +4 more
doaj +1 more source