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Random amplified polymorphic DNA technique for identification and differentiation of old world Leishmania species. [PDF]
The American journal of tropical medicine and hygiene, 2002 The random amplified polymorphic DNA technique may be used to explore parasite DNA polymorphisms. We assessed its applicability to identification of Old World Leishmania species. A set of 6 random decamer primers (Al, A4, A5, A7, A10, and A15) was applied to a panel of DNA from 57 representatives of different Old World Leishmania species.
Guizani Ikram, K. Dellagi, R. B. Ismaïl
semanticscholar +5 more sources
International Journal of Nanomedicine, 2011 Qicai Liu1,2, Ailin Liu3,4, Feng Gao5, Shaohuang Weng3,4, Guangxian Zhong3,4, Jingfeng Liu6, Xinhua Lin3,4, Jian-hua Lin7, Xuhai Chen81Department of Laboratory Medicine, Fujian Medical University, Fuzhou, 2Department of Gene Diagnosis, Fujian Medical ...
Liu Q +8 more
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Aquaculture Research, 1998 Random amplified polymorphic DNA (RAPD) analysis was applied to three varieties of common carp, Cyprinus carpio L.: Xingguo red carp, German mirror carp and Russian mirror carp.
Z. Dong, E. Zhou
semanticscholar +4 more sources
Resistance of Lactobacillus Sp. SKG 34 in The Digestive Tract of Rats Detected Using Random Amplified Polymorphic DNA Technique [PDF]
BIO Web of ConferencesIn recent years, interest in probiotics, particularly Lactobacillus strains like Lactobacillus sp. SKG34, isolated from Sumbawa mare milk, has surged due to their potential health benefits.
Nocianitri Komang Ayu +2 more
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Amplification of bacterial DNA bound on clay minerals by the random amplified polymorphic DNA (RAPD) technique [PDF]
FEMS Microbiology Ecology, 1996 Chromosomal DNA from Bacillus subtilis, bound on the clay minerals, montmorillonite (Wyoming (W) and Apache County (Ap)) and kaolinite (K), was subjected to the random amplified polymorphic DNA (RAPD) technique. DNA bound on the clays was not amplified with 0.625, 1.875, 6.25, and 12.5 U of Taq DNA polymerase, but amplification occurred when the clay ...
Vettori +9 more
+7 more sources
Random amplified polymorphic DNA technique for the identification of Trichinella species
Parasitology, 1993 SUMMARYThe random amplified polymorphic DNA (RAPD) technique was successfully used to produce genetic fingerprints distinguishing between Trichinella spiralis and Trichinella britovi. The same patterns were obtained from purified and crude DNA preparations of pooled and single muscle larvae.
C. Bandi +4 more
semanticscholar +5 more sources
IDENTIFICATION OF GYMNEMA SPECIES BY RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE AND CHLOROPLAST trnK GENE [PDF]
Journal of microbiology, biotechnology and food sciences, 2014 Gymnema is one of the important anti-diabetic medicinal plants used from ancient times and is commonly known as ‘sugar killer’. Most of its species have been used in many applications in Indian traditional medicine. Nevertheless, their efficiency is critically dependent on the use of the correct material.
Subburaj Jayachandran +2 more
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A new genetic analysis of Ficus spp. By HAT-Random amplified Polymorphic DNA Technique
Procedia Engineering, 2012 AbstractWe present the genetic variation within 25 species of Ficus was result by using HAT-RAPD technique. Four single arbitrary primers, OPAS 10, OPG 13, OPLO 5 and OPWO 6, which used to amplify DNA polymorphic bands. From the total of 143 bands and 140 bands were polymorphisms.
W. Phromthep
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KAGAKU KOGAKU RONBUNSHU, 1999 Random Amplified Polymorphic DNA (RAPD) 法は遺伝子多型の検出にしばしば利用される手法である.従来のRAPD法によるパターン認識は, 目視による評価に依存しているため, 得られる結果の信頼性が問題となる.このような人為的なエラーを解決するため, 筆者らは計算機による画像処理法とニューラルネットワークによるパターン認識法を導入し, 微生物の種間の差異に基づくRAPDパターンの差異の識別法を開発したので, 本稿において報告する.
Teruyuki Nagamun +2 more
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Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban triatominae [PDF]
Revista do Instituto de Medicina Tropical de São Paulo, 2005 Random amplified polymorphic DNA (RAPD) technique is a simple and reliable method to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of assay. In this study, we analyzed the effect of changing the concentration of primer, magnesium chloride, template DNA and Taq DNA ...
Fraga, Jorge +4 more
openaire +6 more sources