Results 61 to 70 of about 8,202 (265)

CULTURAL, MORPHOLOGICAL, PHYSIOLOGICAL AND MOLECULAR STUDIES ON SOME STREPTOMYCETE ISOLATES [PDF]

Arab Universities Journal of Agricultural Sciences, 2005
In this study, three isolates of Streptomyces isolated from Egyptian soil at Giza Governorate and belonging to red series were identified based on their cultural, morphological and physiological characters.
H Abdel-Fattah
doaj   +1 more source

Single‐Cell Mitochondrial Lineage Tracing Decodes Fate Decision and Spatial Clonal Architecture in Human Hematopoietic Organoids

Advanced Science, EarlyView.
This study repurposes mitochondrial DNA mutations as endogenous barcodes for lineage tracing in human pluripotent stem cell‐derived organoids. Integrated with transcriptomic and spatial data, it reveals NOTCH‐mediated stromal‐progenitor crosstalk orchestrates clonal dynamics and spatial zonation during early hematopoietic development, offering a non ...
Yan Xue, Junhao Su, Yiming Chao, Lu Liu, Xinyi Lin, Yang Xiang, Mun Kay Ho, Zezhuo Su, Junyi Chen, Zhuojuan Luo, Chengqi Lin, Ruibang Luo, Theo Aurich, Jianfeng Wu, Kelvin Sin Chi Cheung, Yuanhua Huang, Joshua W. K. Ho, Ryohichi Sugimura   +17 more
wiley   +1 more source

Simple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes

Journal of Microbiology & Biology Education, 2018
DNA fingerprinting is a major tool in identifying individuals and in evidence matching. However, this technique can be difficult to reproduce in practical classes. Here, we report on distinct PCR profiles obtained when amplifying saliva DNA of a score of
Ana C. Henriques, Paolo De Marco
doaj   +1 more source

Cytokine‐Engineered Chimeric Antigen Receptor‐T Cell Therapy: How to Balance the Efficacy and Toxicity

Advanced Science, EarlyView.
Cytokine‐engineered CAR‐T cells represent a promising immunotherapy against malignancies due to direct tumor killing and potent immunity response. However, significant toxicities, including CRS and ICANS, have restricted clinical applications. How to keep the risk‐benefit balance of the advanced therapy is of great importance for maximizing the benefit
Xinru Zhang, Gulizeba Aimaiti, Yuanye Guan, Yuzhe Sha, Wei Zhou, Jiefeng Shen, Bo Zhao, Wei‐En Yuan   +7 more
wiley   +1 more source

CD147/Basigin: From Integrative Molecular Hub to Translational Therapeutic Target

Advanced Science, EarlyView.
This review conceptualizes CD147 as a fundamental “Energy‐Structure Coupler,” physically bridging metabolic flux (via MCTs) with morphogenetic plasticity (via integrins/MMPs) to drive cancer, infection, and autoimmunity. Addressing the “specificity paradox” that limits current translation, the authors chart a strategic roadmap—spanning logic‐gated ...
Xiang‐Min Yang, Huijie Bian, Zhi‐Nan Chen   +2 more
wiley   +1 more source

Clustering of Brown Planthopper Biotype Based on RAPD-PCR

Hayati Journal of Biosciences, 2005
Random amplified polymorphic DNA (RAPD) technique was used to differentiate two brown planthopper biotypes, that were multiplied in the greenhouse. Ten selected random decamer RAPD primers produced unique DNA band patterns for each individual brown ...
BAHAGIAWATI, HABIB RIJZAANI
doaj  

Assessment of genetic relationship between Klebsiella pneumoniae and Klebsiella oxytoca samples isolated from a dental office

Journal of Venomous Animals and Toxins including Tropical Diseases, 2008
The present study aimed to analyze the genetic similarity between genomic profiles of thirteen Klebsiella oxytoca and seven Klebsiella pneumoniae samples isolated from two different collections carried out in different places of dental offices.
MV Pimenta-Rodrigues, AM Fusco-Almeida, BW Bertoni, RCLR Pietro   +3 more
doaj   +1 more source

Systematic Engineering of Proteases in Saccharopolyspora Spinosa Reveals Synergistic Enhancement of Spinosad Biosynthesis via Substrate Flux Optimization

Advanced Science, EarlyView.
ARTP mutagenesis yielded Saccharopolyspora spinosa mutant D184 with improved extracellular nitrogen utilization. An integrated workflow of protease genetic manipulation, multi‐omics, and rational synergy design pinpointed a pepP‐clpP‐htpX synergistic triangular combination.
Duo Jin, Wangqiong Chen, Danlu Yang, Qing Liu, Baolong Bai, Zirui Dai, Xirong Liu, Zirong Zhu, Jie Rang, Liqiu Xia   +9 more
wiley   +1 more source

Genetic polymorphism among 14 elite Coffea arabica L. cultivars using RAPD markers associated with restriction digestion

Genetics and Molecular Biology, 2003
Knowledge of the genetic variability among genotypes is important for the transfer of useful genes and to maximize the use of available germplasm resources. This study was carried out to assess the genetic variability of 14 elite Coffea arabica cultivars
Tumoru Sera, Paulo Maurício Ruas, Claudete de Fátima Ruas, Leandro Eugênio Cardamone Diniz, Valdemar de Paula Carvalho, Leandro Rampim, Eduardo Augusto Ruas, Sheila Recepute da Silveira   +7 more
doaj   +1 more source

Mepylome: A Point‐of‐Care Tumor Diagnostic Toolkit for Tumor DNA Methylation and Copy Number Analysis

Advanced Intelligent Systems, EarlyView.
DNA methylation and chromosomal copy number profiling have recently become essential for tumor diagnostics. The open‐source tool Mepylome enables this task in clinical routine. It combines several machine learning strategies and allows users to interactively examine respective data through an intuitive graphical interface. Running up to 65 times faster
Jon Brugger, Claus Hultschig, Daniel Baumhoer, Baptiste Ameline, Benjamin Maciej Freyter, Stephan Frank, Jürgen Hench   +6 more
wiley   +1 more source