Results 121 to 130 of about 4,824,315 (311)

Resultados preliminares de la evaluación del primer sistema látex en el diagnóstico rápido de la leptospirosis humana en Cuba Preliminary results of the evaluation of the first latex system in the rapid diagnosis of human leptospirosis in Cuba

Revista Cubana de Higiene y Epidemiología, 2005
Se elaboró un látex de aglutinación de producción nacional (leptospira - látex - IPK) para el diagnóstico rápido de la leptospirosis humana. La dilución óptima de trabajo del antígeno termorresistente (TR) acoplado a las partículas de látex fue de 1/80 ...
Ana Margarita Obregón Fuentes, Carmen Fernández Molina, María del Carmen Batlle, José Rodríguez, Islay Rodríguez, Idalmis Hernández   +5 more
doaj  

An overview of rapid non-culture-based techniques in various clinical specimens for the laboratory diagnosis of Talaromyces marneffei

Frontiers in Cellular and Infection Microbiology
Talaromyces marneffei (T. marneffei) is a temperature-dependent biphasic deep opportunistic infectious fungus that primarily affects individuals with advanced HIV disease and other immunocompromised populations.
Huamei Wei, Huamei Wei, Patcharin Thammasit, Artid Amsri, Artid Amsri, Kritsada Pruksaphon, Fenglian Deng, Joshua D. Nosanchuk, Sirida Youngchim   +8 more
doaj   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Molecular epidemiology and phylogenetic analysis of influenza viruses A (H3N2) and B/Victoria during the COVID-19 pandemic in Guangdong, China

Infectious Diseases of Poverty
Background Non-pharmaceutical measures and travel restrictions have halted the spread of coronavirus disease 2019 (COVID-19) and influenza. Nonetheless, with COVID-19 restrictions lifted, an unanticipated outbreak of the influenza B/Victoria virus in ...
Zhiqi Zeng, Yong Liu, Wenxiang Jin, Jingyi Liang, Jinbin Chen, Ruihan Chen, Qianying Li, Wenda Guan, Lixi Liang, Qiubao Wu, Yuanfang Lai, Xiaoyan Deng, Zhengshi Lin, Chitin Hon, Zifeng Yang   +14 more
doaj   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Rapid and specific detection of Streptococcus suis serotype 2 using a RPA–PfAgo system coupled with fluorescence and lateral flow dipstick

Frontiers in Veterinary Science
ObjectiveTo develop and validate dual detection platforms integrating recombinase polymerase amplification (RPA) with Pyrococcus furiosus Argonaute (PfAgo) for the rapid and specific identification of Streptococcus suis serotype 2.MethodsThe conserved ...
Kexin Wang, Kexin Wang, Xujie Zhao, Xujie Zhao, Jingjing Li, Jingjing Li, Mingzhu Zhou, Mingzhu Zhou, Bingze Jiao, Bingze Jiao, Yilin Bai, Zhanwei Teng, Zhanwei Teng, Meinan Chang, Meinan Chang, Yueyu Bai, Jianhe Hu, Jianhe Hu, Ke Ding, Ke Ding, Xiaojing Xia, Xiaojing Xia   +21 more
doaj   +1 more source

TRPML1 agonist ML‐SA5 attenuates pulmonary fibroblast activation by suppressing mTOR and restoring autophagic flux

FEBS Open Bio, EarlyView.
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao, Xiaoting Kang, Lulu Meng, Shaoxuan Zhang, Qiujie Li, Jinyu Zhou, Mengyue Xue, Chu Li, Hua Zhu, Pengzhou Hang, Jing Zhao   +10 more
wiley   +1 more source

The clinical utility of Nanopore 16S rRNA gene sequencing for direct bacterial identification in normally sterile body fluids

Frontiers in Microbiology
The prolonged incubation period of traditional culture methods leads to a delay in diagnosing invasive infections. Nanopore 16S rRNA gene sequencing (Nanopore 16S) offers a potential rapid diagnostic approach for directly identifying bacteria in infected
Hiu-Yin Lao, Lily Lok-Yee Wong, Yan Hui, Timothy Ting-Leung Ng, Chloe Toi-Mei Chan, Hazel Wing-Hei Lo, Miranda Chong-Yee Yau, Eddie Chi-Man Leung, River Chun-Wai Wong, Alex Yat-Man Ho, Kam-Tong Yip, Jimmy Yiu-Wing Lam, Viola Chi-Ying Chow, Kristine Shik Luk, Tak-Lun Que, Franklin Wang Ngai Chow, Gilman Kit-Hang Siu   +16 more
doaj   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source