Results 191 to 200 of about 489,800 (291)

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Evaluation of GABAergic and Glutamatergic Levels in the Dorsal Anterior Cingulate Cortex of Patients With Bipolar Disorder: A Cross-Sectional ¹H-MRS Study. [PDF]

Neuropsychopharmacol Rep
Moriyama S, Noda Y, Honda S, Edden RAE, Takahashi K, Saito K, Taniguchi K, Wada M, Tsugawa S, Tobari Y, Huh S, Nakane Y, Homma S, Matsui M, Koike S, Mimura M, Uchida H, Nakajima S.   +17 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Using a Quality Improvement Priority Metric to Promote Uniformly High-Quality Care: An Illustrative Example Using Child HCAHPS Survey Data. [PDF]

J Patient Exp
Quigley DD, Elliott MN, Slaughter ME, Hays RD.   +3 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Psychometric Properties of the Mixed State Severity Index for Patients With Mood Disorder. [PDF]

Psychiatry Investig
Myung W, Yu H, Kang HS, Lee D, Jang J, Lee J, Yoon J, Park YS, Ryoo HA, Kim YR, Park KH, Lee CW, Jang Y, Kim K, Lee N, Hong S, Won HH, Ha TH, Park J.   +18 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Active disambiguation guides inferring controllability and cause in social interactions. [PDF]

Nat Commun
Spiering L, Trier HA, O'Reilly JX, Kolling N, Wittmann MK, Rushworth MFS, Scholl J.   +6 more
europepmc   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source

Effect of the rs7858836 Single-Nucleotide Polymorphisms of the ASTN2 Gene on Pain-Related Phenotypes in Japanese Women Who Underwent Laparoscopic Gynecologic Surgery. [PDF]

Neuropsychopharmacol Rep
Inoue R, Nishizawa D, Okahara S, Hara A, Okada H, Hasegawa J, Ebata Y, Nakayama K, Hayashida M, Ikeda K.   +9 more
europepmc   +1 more source