Results 81 to 90 of about 168,562 (302)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Rational Bubbles in Stock Prices? [PDF]

open access: yes
This paper reports empirical tests for the existence of rational bubbles in stock prices. The analysis focuses on a familiar model that defines market fundamentals to be the expected present value of dividends, discounted at a constantrate, and defines a
Herschel I. Grossman, Behzad T. Diba
core  

An intergenerational theory of the consumption function [PDF]

open access: yes, 1991
This thesis presents a theory of the consumption function called the 'Inter- Generational Hypothesis' (IGH). The theory starts from the premise that individuals derive utility not only from their own consumption, but also from the welfare of their ...
Parker, S.C, Parker, Simon C
core  

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Multi-skyrmion solutions of a sixth order skyrme model [PDF]

open access: yes, 2001
In this Thesis, we study some of the classical properties of an extension of the Skyrme model defined by adding a sixth order derivative term to the Lagrangian.
Floratos, Ioannis, Floratos, I.
core  

Computation of Inner-Outer Factorizations of Rational Matrices

open access: yes, 1997
In this paper we propose a new numerically reliable computational approach to determine the inner-outer factorization of a rational transfer matrix G of a linear descriptor system.
A. Varga, Andras Varga, Varga, A.
core   +1 more source

Rational Transfer Function Model for a Double-Pipe Parallel-Flow Heat Exchanger

open access: yes, 2020
Transfer functions of typical heat exchangers, resulting from their partial differential equations, usually contain irrational functions which quite accurately describe the spatio-temporal nature of the processes occurring therein.
Krzysztof Bartecki
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

Higher-order perturbation solutions to dynamic, discrete-time rational expectations models [PDF]

open access: yes
We present an algorithm and software routines for computing nth order Taylor series approximate solutions to dynamic, discrete-time rational expectations models around a nonstochastic steady state.
Andrew Levin   +2 more
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

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