Results 71 to 80 of about 487,788 (325)

Genome-wide characterization leading to simple sequence repeat (SSR) markers development in Shorea robusta

Functional & Integrative Genomics, 2023
Tropical rainforests in Southeast Asia are enriched by multifarious biota dominated by Dipterocarpaceae. In this family, Shorea robusta is an ecologically sensitive and economically important timber species whose genomic diversity and phylogeny remain ...
G. Mishra, R. Meena, R. Kant, S. Pandey, H. Ginwal, M. Bhandari   +5 more
semanticscholar   +1 more source

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources

Targeted nanopore sequencing by real-time mapping of raw electrical signal with UNCALLED

Nature Biotechnology, 2020
Conventional targeted sequencing methods eliminate many of the benefits of nanopore sequencing, such as the ability to accurately detect structural variants or epigenetic modifications.
S. Kovaka, Yunfan Fan, Bohan Ni, W. Timp, M. Schatz   +4 more
semanticscholar   +1 more source

baseLess: lightweight detection of sequences in raw MinION data

bioRxiv, 2022
With its candybar form factor and low initial investment cost, the MinION brought affordable portable nucleic acid analysis within reach. However, translating the electrical signal it outputs into a sequence of bases still requires high-end computer ...
Ben Noordijk, R. Nijland, V. Carrión, J. Raaijmakers, D. de Ridder, C. de Lannoy   +5 more
semanticscholar   +1 more source

First report of bacterial community from a Bat Guano using Illumina next-generation sequencing

Genomics Data, 2015
V4 hypervariable region of 16S rDNA was analyzed for identifying the bacterial communities present in Bat Guano from the unexplored cave — Pnahkyndeng, Meghalaya, Northeast India.
Surajit De Mandal, Zothansanga, Amritha Kumari Panda, Satpal Singh Bisht, Nachimuthu Senthil Kumar   +4 more
doaj   +1 more source

Diagnostic applications of next generation sequencing: working towards quality standards [PDF]

, 2012
Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Adey, Andrea Gehring, Anna Benet-Pagès, Bainbridge, Bentley, Carsten Bergmann, Clark, Clement, Ding, Dohm, Gerlinger, Gregory, Greif, Greif, Hanno Jörn Bolz, Hanns-Georg Klein, Harismendy, Ina Vogl, Jiang, Johansson, Kaimo Hirv, Kalari, Klaus H. Metzeler, Koboldt, Li, Lister, Loman, Mamanova, Manfred Stuhrmann, Mardis, Marius Kuhn, Mertes, Meyerson, Minoche, Nakamura, Philipp A. Greif, Robinson, Rothberg, Saskia Biskup, Sebastian H. Eck, Shendure, Stefan Kotschote, Stratton   +42 more
core   +1 more source

AAA+ protein unfoldases—the Moirai of the proteome

FEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

Initial genome sequencing of the sugarcane CP 96-1252 complex hybrid [version 1; referees: 2 approved]

F1000Research, 2017
The CP 96-1252 cultivar of sugarcane is a complex hybrid of commercial importance. DNA was extracted from lab-grown leaf tissue and sequenced. The raw Illumina DNA sequencing results provide 101 Gbp of genome sequence reads. The dataset is available from
Jason R. Miller, Kari A. Dilley, Derek M. Harkins, Manolito G. Torralba, Kelvin J. Moncera, Karen Beeri, Karrie Goglin, Timothy B. Stockwell, Granger G. Sutton, Reed S. Shabman   +9 more
doaj   +1 more source

Genome analysis of biosurfactant producing bacterium, Bacillus tequilensis.

PLoS ONE, 2023
Bioremediation is crucial for recuperating polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation.
Anuraj Nayarisseri, Sanjeev Kumar Singh
doaj   +1 more source

SECAPR—a bioinformatics pipeline for the rapid and user-friendly processing of targeted enriched Illumina sequences, from raw reads to alignments

PeerJ, 2018
Evolutionary biology has entered an era of unprecedented amounts of DNA sequence data, as new sequencing technologies such as Massive Parallel Sequencing (MPS) can generate billions of nucleotides within less than a day.
Tobias Andermann, Á. Cano, Alexander Zizka, C. Bacon, A. Antonelli   +4 more
semanticscholar   +1 more source