A cytogenetic investigation was performed in eight species of the spittlebug genus Philaenus using silver-NOR (AgNOR)-banding and fluorescence in situ hybridization (FISH) with 18S rDNA and (TTAGG)n telomeric probes. This is the first application of FISH
Anna MARYAŃSKA-NADACHOWSKA +2 more
doaj +1 more source
Marine nematode taxonomy in the DNA age: the present and future of molecular tools to access their biodiversity [PDF]
Molecular taxonomy is one of the most promising yet challenging fields of biology. Molecular markers such as nuclear and mitochondrial genes are being used in a variety of studies surveying marine nematode taxa.
da Silva, Maria Cristina +7 more
core +2 more sources
Molecular characterization and phylogeny of the entomopathogenic fungus
We characterized 23 isolates of the entomopathogenic fungus Aschersonia spp. from Mexico, Brazil, Guyana, Trinidad, Venezuela, Columbia, Florida, Malaysia, Thailand, Japan, Philippines, Java and South India using RAPD markers.
Miroslav Oborník +3 more
doaj +1 more source
Identification of Host-Specific Bacteroidales 16S rDNA Sequences from Human Sewage and Ruminant Feces [PDF]
The need to identify the source of fecal contamination of water has led to the development of various fecal source identification methods, a field known as microbial source tracking (MST). One promising method of MST focuses on fecal members of the order
Allsop +25 more
core +1 more source
The use of DNA sequences analysis has been an important mean to distinguish and to identify populations of organisms at different levels. By molecular markers several complex organisms have been successful detected in plants for distinct aims.
Enia Mara de Carvalho +4 more
doaj +1 more source
Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences [PDF]
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Speksnijder, A.G.C.L. +5 more
core +3 more sources
The present study focused on the evolution of the karyotype in 21 taxa of the genus Isophya, which was done by mapping the location on the chromosomes of ribosomal RNA (rRNA) coding genes using fluorescence in situ hybridization (FISH) with an 18S rDNA ...
Beata GRZYWACZ +4 more
doaj +1 more source
Chlamydia psittaci infection in canaries heavily infested by Dermanyssus gallinae [PDF]
Dermanyssus gallinae is a haematophagous ectoparasite responsible for anemia, weight loss, dermatitis and a decrease in egg production. Dermanyssus gallinae may play a role in the modulation of the host immune system, maybe predisposing the host to some ...
Cafiero, Maria Assunta +5 more
core +2 more sources
Molecular variations in Lenzites species collected from Nigeria and other parts of the world using Internal Transcribed Spacers (ITS) regions of Ribosomal RNA [PDF]
The phylogeny of six specimens of Lenzites species collected from Akure Nigeria was studied using molecular data obtained by sequencing the internal transcribed spacers (ITS) regions of ribosomal RNA using universal primers (ITS4 and ITS5).
doaj +1 more source
Service evaluation to establish the sensitivity, specificity and additional value of broad-range 16S rDNA PCR for the diagnosis of infective endocarditis from resected endocardial material in patients from eight UK and Ireland hospitals [PDF]
Infective endocarditis (IE) can be diagnosed in the clinical microbiology laboratory by culturing explanted heart valve material. We present a service evaluation that examines the sensitivity and specificity of a broad-range 16S rDNA polymerase chain ...
Alshafi, K +8 more
core +1 more source

