Results 21 to 30 of about 34,349 (240)

Development of a Reagent Kit for the Quantitative Determination of Hepatitis B Virus (HBV) DNA in Clinical Material by PCR with Hybridization-Fluorescence Detection

Эпидемиология и вакцинопрофилактика, 2023
Relevance. Hepatitis B virus (HBV) is one of the most common viral infections affecting people worldwide and can lead to chronic hepatitis, cirrhosis and hepatocellular carcinoma. Currently 3% of the world's population are infected with hepatitis B virus
O. N. Zhigaleva, S. G. Mardanly, T. Yu. Gashenko, I. I. Ermolaev   +3 more
doaj   +1 more source

External Quality Assessment for the Detection of Measles Virus by Reverse Transcription-PCR Using Armored RNA. [PDF]

PLoS ONE, 2015
In recent years, nucleic acid tests for detection of measles virus RNA have been widely applied in laboratories belonging to the measles surveillance system of China.
Dong Zhang, Yu Sun, Tingting Jia, Lei Zhang, Guojing Wang, Rui Zhang, Kuo Zhang, Guigao Lin, Jiehong Xie, Lunan Wang, Jinming Li   +10 more
doaj   +1 more source

Evaluation of the diagnostic efficacy of a reagent kit for <i>in vitro</i> diagnosis of West Nile fever using reverse transcription polymerase chain reaction with fluorescent probe-based detection

Biological Products. Prevention, Diagnosis, Treatment, 2023
West Nile fever is a vector-borne zoonotic arbovirus infection with natural foci. Its clinical course is similar to that of acute febrile syndrome, and severe cases may result in neuroinvasive disease. Several genetic lineages (1, 2, and 4) of the West Nile virus (WNV) with different pathogenicity for humans are circulating in the Russian Federation ...
E. V. Prokhvatilova, G. A. Tkachenko, A. A. Baturin, L. I. Belitskaya, A. V. Toporkov   +4 more
openaire   +2 more sources

AUTOANTIBODIES TO GLUTAMIC ACID DECARBOXYLASE AS A PATHOGENETIC MARKER OF TYPE I DIABETES MELLITUS

Медицинская иммунология, 2014
. A new method of enzyme-linked immunosorbent assay (in solid-phase ELISA format) has been developed to determine concentrations of autoantibodies to glutamic acid decarboxylase, as well as an evidencebased methodology is proposed for its medical ...
N. V. Piven, L. N. Lukhverchyk, A. I. Burakovsky, N. V. Polegenkaya, M. V. Karpovich   +4 more
doaj   +1 more source

GroEL Chaperonin-Based Assay for Early Diagnosis of Scrub Typhus

Diagnostics, 2022
A point-of-care diagnostic for early and rapid diagnosis of scrub typhus caused by Orientia tsutsugamushi is required for prompt and proper treatment of patients presenting with undifferentiated febrile illnesses.
Nitaya Indrawattana, Pisinee Aiumurai, Nawannaporn Sae-lim, Watee Seesuay, Onrapak Reamtong, Manas Chongsa-nguan, Wanpen Chaicumpa, Nitat Sookrung   +7 more
doaj   +1 more source

A fluorescent microspheres-based microfluidic test system for the detection of immunoglobulin G to SARS-CoV-2 [PDF]

Клиническая практика, 2023
Background: The pandemic of the new coronavirus infection, COVID-19, is currently ongoing in the world. Over the years, the pathogen, SARS-CoV-2, has undergone a series of mutational genome changes, which has led to the spread of various genetic variants
Ruslan I. Shakurov, Yaroslav D. Shansky, Kirill A. Prusakov, Svetlana V. Sizova, Stepan P. Dudik, Lyudmila V. Plotnikova, Valentin A. Manuvera, Dmitry V. Klinov, Vassili N. Lazarev, Julia A. Bespyatykh, Dmitriy V. Basmanov   +10 more
doaj   +1 more source

Evaluation of a commercial ELISA kit for the detection of anti-dengue IgM in serum of patients from a hospital in the north of Peru

Revista Electrónica Dr. Zoilo E. Marinello Vidaurreta, 2021
Background: capture-Igm (immunoglobulin) ELISA (enzyme-linked immunosorbent assay) is the main immunoenzymatic assay used in the diagnosis of dengue, for this purpose there are several commercial tests with different output.Objective: to evaluate the ...
Christian Junnior Campos-Monteza, Heber Silva-Díaz, Danny Omar Suclupe-Campos, Franklin Rómulo Aguilar–Gamboa   +3 more
doaj  

Evaluation of miniaturized Illumina DNA preparation protocols for SARS-CoV-2 whole genome sequencing.

PLoS ONE, 2023
The global pandemic caused by SARS-CoV-2 has increased the demand for scalable sequencing and diagnostic methods, especially for genomic surveillance.
Sureshnee Pillay, James Emmanuel San, Derek Tshiabuila, Yeshnee Naidoo, Yusasha Pillay, Akhil Maharaj, Ugochukwu J Anyaneji, Eduan Wilkinson, Houriiyah Tegally, Richard J Lessells, Cheryl Baxter, Tulio de Oliveira, Jennifer Giandhari   +12 more
doaj   +2 more sources

Diagnostic performance evaluation of urine HIV-1 antibody rapid test kits in a real-life routine care setting in China

BMJ Open
Objectives To evaluate the diagnostic performance of urine HIV antibody rapid test kits in screening diverse populations and to analyse subjects’ willingness regarding reagent types, purchase channels, acceptable prices, and self-testing.Designs ...
Huanhuan Chen, Qiuying Zhu, Guanghua Lan, Yuhua Ruan, Mei Lin, Shujia Liang, Jianjun Li, Huaxiang Lu, Guangjie Tan, Xianwu Pang, Xianmin Ge, Yunxian Huang, Zhenqiang Chen, Shizhen Zhang, Wenlong Cai   +14 more
doaj   +1 more source

DEVELOPMENT OF HEPATITIS E 3 GENOTYPE RECOMBINANT PROTEIN CAPSID OF: CLONING, EXPRESSION, PURIFICATION, EVALUATION OF THE ANTIGENIC PROPERTIES

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Aim. The development of the hepatitis E virus (HEV) genotype 3 recombinant capsid protein. Materials and methods. E.coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological,
G. I. Alatortseva, A. V. Sidorov, L. N. Nesterenko, L. N. Luhverchik, V. V. Dotsenko, I. I. Amiantova, M. V. Zhukina, V. Yu. Kabargina, A. V. Milovanova, D. S. Vorobev, Yu. I. Ammur, M. I. Mikhailov, K. K. Kyuregyan, E. Yu. Malinnikova, S. V. Zhavoronok, V. M. Blinov, V. V. Zverev   +16 more
doaj   +1 more source