Results 111 to 120 of about 19,571,554 (293)
Real-time PCR in the microbiology laboratory [PDF]
Clinical Microbiology and Infection, 2004 Use of PCR in the field of molecular diagnostics has increased to the point where it is now accepted as the standard method for detecting nucleic acids from a number of sample and microbial types. However, conventional PCR was already an essential tool in the research laboratory.
openaire +5 more sources
Molecular Oncology, EarlyView.High‐risk bladder cancer is typically treated with Bacillus Calmette‐Guérin (BCG), but 30–40% of patients relapse. No FDA‐ or CE‐approved biomarkers currently predict or prognosticate BCG failure. We systematically reviewed the literature and identified 72 eligible studies, revealing several promising biomarkers associated with BCG treatment response ...
Rui Ribeiro‐Pereira +7 more
wiley +1 more source
Real-time PCR: Concept and Application in Livestock
International Journal of Bio-Resource and Stress Management, 2023 Real-time PCR, also called quantitative PCR or RT-qPCR, is a technique which provide a simple and elegant method for determining the amount of a target sequence or gene that is present in a sample. It is a gold standard method for accurate and sensitive
Mahesh S. Dige +3 more
doaj
A Real-time Service Oriented Infrastructure [PDF]
, 2010 The advancements in distributed computing have driven the emergence of service-based infrastructures that allow for on-demand provision of ICT assets. Taking into consideration the complexity of distributed environments, significant challenges exist in ...
Michael Boniface +26 more
core +1 more source
Molecular Oncology, EarlyView.In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico +5 more
wiley +1 more source
One HealthBackground: The family Rickettsiaceae, especially the genus Rickettsia, refers to obligate intracellular pathogens responsible for zoonotic diseases such as rickettsiosis.
Abdolghaffar Ownagh +2 more
doaj +1 more source
Primers with 5′ flaps improve real-time PCR
BioTechniques, 2007 Primers that contain portions noncomplementary to the target region are usually used to add to the PCR product a utility sequence such as a restriction site or a universal probe binding site.
Irina Afonina +5 more
doaj +1 more source
Molecular Oncology, EarlyView.This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault +12 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Real-time PCR primer sequences.
, 2017 Real-time PCR primer sequences.
Xiao-Qiu Zhou (387187) +10 more
core +1 more source