Results 101 to 110 of about 20,355,262 (262)
One HealthBackground: The family Rickettsiaceae, especially the genus Rickettsia, refers to obligate intracellular pathogens responsible for zoonotic diseases such as rickettsiosis.
Abdolghaffar Ownagh +2 more
doaj +1 more source
Enumeration of Mycobacterium leprae using real-time PCR.
PLoS Neglected Tropical Diseases, 2008 Mycobacterium leprae is not cultivable in axenic media, and direct microscopic enumeration of the bacilli is complex, labor intensive, and suffers from limited sensitivity and specificity. We have developed a real-time PCR assay for quantifying M. leprae
Richard W Truman +5 more
doaj +1 more source
Molecular Oncology, EarlyView.Loss of NAPRT promotes lung tumor initiation and growth through a noncanonical mechanism, independent of its role in NAD+ biosynthesis. Mechanistically, NAPRT depletion activates the mTORC2‐driven AKT/β‐catenin signaling axis to enhance clonogenic and invasive phenotypes. Furthermore, lung‐specific Naprt deletion significantly increases tumor burden in
Myung Joon Oh +11 more
wiley +1 more source
PCR primers for quantitative real-time PCR.
, 2013 PCR primers for quantitative real-time PCR.
Delphine M. Vauthay (298928) +9 more
core +1 more source
Real-time PCR: Concept and Application in Livestock
International Journal of Bio-Resource and Stress Management, 2023 Real-time PCR, also called quantitative PCR or RT-qPCR, is a technique which provide a simple and elegant method for determining the amount of a target sequence or gene that is present in a sample. It is a gold standard method for accurate and sensitive
Mahesh S. Dige +3 more
doaj
Molecular Oncology, EarlyView.In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico +5 more
wiley +1 more source
Real-time PCR primer sequences 1.
, 2019 Real-time PCR primer sequences 1.
Xiao-Qiu Zhou (387187) +11 more
core +1 more source
Primers with 5′ flaps improve real-time PCR
BioTechniques, 2007 Primers that contain portions noncomplementary to the target region are usually used to add to the PCR product a utility sequence such as a restriction site or a universal probe binding site.
Irina Afonina +5 more
doaj +1 more source
Molecular Oncology, EarlyView.This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault +12 more
wiley +1 more source
Molecular Oncology, EarlyView.We established the avian chorioallantoic membrane (CAM) assay as a scalable in vivo model for studying circulating tumor cells (CTCs). Human gastrointestinal tumors spontaneously released genetically validated CTCs that were detected across multiple platforms, demonstrating that the CAM model provides an accessible tool for investigating early cancer ...
Dennis Roth +17 more
wiley +1 more source