Results 81 to 90 of about 1,770,911 (287)

Epidemiology of Plasmodium infections in Flores Island, Indonesia using real-time PCR [PDF]

, 2013
BACKGROUND:\ud DNA-based diagnostic methods have been shown to be highly sensitive and specific for the detection of malaria. An 18S-rRNA-based, real-time polymerase chain reaction (PCR) was used to determine the prevalence and intensity of Plasmodium ...
Brienen, Eric A. T., Hamid, Firdaus, Kaisar, Maria M. M., Luty, Adrian J. F., Sartono, Erliyani, Supali, Taniawati, van Lieshout, Lisette, Verweij, Jaco J., Wammes, Linda J, Wiria, Aprilianto E., Yazdanbakhsh, Maria   +10 more
core   +2 more sources

Democratizing the Real-Time PCR [PDF]

Clinical Chemistry, 2017
Featured Article: Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 1997;22:130–1, 134–8.3 After the development of the PCR in the second half of the1980s and demonstration of real-time analysis in 1992 (1), it was not clear how the technology would unfold.
openaire   +2 more sources

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Comparison of immunoassay and real-time PCR methods for the detection of Jembrana disease virus infection in Bali cattle [PDF]

, 2009
A sensitive diagnostic assay for the detection of infections with the bovine lentivirus Jembrana disease virus (JDV) is required in Indonesia to control the spread of Jembrana disease. Immunoassays are used routinely but are compromised by cross-reactive
Desport, M., Hartaningsih, N., Lewis, J.R., McNab, T., Tenaya, I.W., Wilcox, G.E.   +5 more
core   +2 more sources

RIPK4 function interferes with melanoma cell adhesion and metastasis

Molecular Oncology, EarlyView.
RIPK4 promotes melanoma growth and spread. RIPK4 levels increase as skin lesions progress to melanoma. CRISPR/Cas9‐mediated deletion of RIPK4 causes melanoma cells to form less compact spheroids, reduces their migratory and invasive abilities and limits tumour growth and dissemination in mouse models.
Norbert Wronski, Sławomir Lasota, Ewelina Madej, Anna A. Brożyna, Małgorzata Szczygieł, Agnieszka Harazin‐Lechowska, Jan Czerbniak, Janusz Rys, Jaroslaw Czyz, Agnieszka Wolnicka‐Glubisz   +9 more
wiley   +1 more source

Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA

Journal of Infection in Developing Countries, 2017
Introduction: The purpose of this study was to evaluate and compare two manual isolation and real-time (RT) polymerase chain reaction (PCR) kits (RTA RT-PCR with RTA isolation kit and Artus RG RT-PCR with QIAamp isolation kit) for molecular diagnosis of
Sibel Aydogan, Ziya Cibal Acikgoz, Aysegul Gozalan, Fisun Kirca, Tuba Muderris, Elife Ozturk   +5 more
doaj   +1 more source

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source

Agreement between two real-time commercial PCR kits and an in-house real-time PCR for diagnosis of mucormycosis

Microbiology Spectrum
Mucormycosis is a severe and emerging invasive fungal infection associated with high mortality rates. Early diagnosis is crucial for initiating specific antifungal treatment, with molecular tools currently representing the most efficient diagnostic ...
Lovanirina Clémencia Rafanomezantsoa, Estelle Sabourin, Nadia Guennouni Sebbouh, Emilie Sitterlé, Nada Ben Halima, Yannick Sonjah Raveloarisaona, Gilles Quesne, Eric Dannaoui, Marie-Elisabeth Bougnoux   +8 more
doaj   +1 more source

Real-time PCR applications for diagnosis of leishmaniasis

Parasites & Vectors, 2018
Leishmaniasis is a vector-borne disease caused by many Leishmania species, which can infect both humans and other mammals. Leishmaniasis is a complex disease, with heterogeneous clinical manifestations ranging from asymptomatic infections to lesions at ...
Luca Galluzzi, Marcello Ceccarelli, Aurora Diotallevi, Michele Menotta, Mauro Magnani   +4 more
doaj   +1 more source

Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR. [PDF]

, 2015
BACKGROUND: Noninvasive genotyping of fetal RHD (Rh blood group, D antigen) can prevent the unnecessary administration of prophylactic anti-D to women carrying RHD-negative fetuses. We evaluated laboratory methods for such genotyping.
Avent, ND, Hollands, HJ, Kiernan, M, Madgett, TE, Roberts, LA, Sillence, KA, Thompson, HP, Welch, CR   +7 more
core   +2 more sources