Results 61 to 70 of about 19,571,554 (293)

Multicolor combinatorial probe coding for real-time PCR. [PDF]

PLoS ONE, 2011
The target volume of multiplex real-time PCR assays is limited by the number of fluorescent dyes available and the number of fluorescence acquisition channels present in the PCR instrument.
Qiuying Huang, Linlin Zheng, Yumei Zhu, Jiafeng Zhang, Huixin Wen, Jianwei Huang, Jianjun Niu, Xilin Zhao, Qingge Li   +8 more
doaj   +1 more source

Reference genes in real-time PCR [PDF]

Journal of Applied Genetics, 2013
This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name.
Bartłomiej Kozera, Marcin Rapacz
openaire   +2 more sources

Transcriptional profiling of circulating extracellular vesicles from prebiopsy prostate cancer patients

Molecular Oncology, EarlyView.
RNA profiling of circulating extracellular vesicles (EVs) from blood samples of men undergoing prostate biopsy identifies transcripts associated with clinically significant prostate cancer. Integrative analysis with public tumor datasets links EV‐derived gene signatures to tumor stage and progression‐free survival, highlighting CASP3, XRCC2, and RIT1 ...
Stefan Werner, Pierre Tennstedt, Randi C. Pose, Christian Müller, Katharina Besler, Svenja Schneegans, Malik Alawi, Marie C. Roesch, Sven Peine, Desiree Bonci, Joanna Budna‐Tukan, Evi Lianidou, Catherine Alix‐Panabières, Derya Tilki, Klaus Pantel   +14 more
wiley   +1 more source

Stimulator of interferon genes agonist augmented antitumor immunity of osimertinib in Egfr‐mutated lung cancer

Molecular Oncology, EarlyView.
Combining osimertinib with the STING agonist ADU‐S100 activates innate and adaptive immunity to overcome the non‐inflamed microenvironment of Egfr‐mutant lung cancer. This combination increases NK and CD8+ T‐cell infiltration, associated with activation of the STING‐IRF3 pathway and local immunogenic cell death.
Jun Nishimura, Tadahiro Kuribayashi, Johannes Brägelmann, Sachi Okawa, Masataka Taoka, Shunta Mori, Tomoka Nishimura, Takaaki Tanaka, Go Makimoto, Kiichiro Ninomiya, Kammei Rai, Eiki Ichihara, Ryohei Katayama, Katsuyuki Hotta, Masahiro Tabata, Yosuke Togashi, Yoshinobu Maeda, Martin L. Sos, Katsuyuki Kiura, Kadoaki Ohashi   +19 more
wiley   +1 more source

Effects of Chiral Gold Nanoflowers on Polymerase Chain Reaction

Nano Biomedicine and Engineering, 2011
In this paper, we reported that chiral gold nanoflowers with the concentration of 0.08 mM can enhance the specificity of polymerase chain reaction (PCR) and eliminate unspecific products, and the efficiency of PCR declines in some extent and the melt ...
Xiao Zhi, Xin Zhang, Peng Huang, Daxiang Cui   +3 more
doaj   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

PCR em tempo real para diagnóstico da leucose enzoótica bovina Enzootic bovine leukosis real time PCR

Ciência Rural, 2012
O objetivo deste trabalho foi realizar a validação de uma reação em cadeia da polimerase em tempo real com o sistema Plexor® (qPCR) para o diagnóstico da Leucose Enzoótica Bovina (LEB), por meio da comparação com testes de diagnóstico recomendados pela ...
Natanael Lamas Dias, Antônio Augusto Fonseca Júnior, Daniel Sobreira Rodrigues, Marcelo Fernandes Camargos   +3 more
doaj  

Detection of Leishmania infantum in Lutzomyia longipalpis captured in Campo Grande, MS

Revista Brasileira de Parasitologia Veterinária
Leishmaniasis is a zoonotic disease caused by protozoa of the genus Leishmania (Ross, 1903) and is the focus of considerable attention in human and veterinary medicine.
Rodrigo Casquero Cunha, Renato Andreotti, Marlon Cezar Cominetti, Elaine Araújo Silva   +3 more
doaj   +1 more source

Real-time PCR: A rapid and sensitive method for diagnosis of dermatophyte induced onychomycosis, a comparative study

Alexandria Journal of Medicine, 2016
Onychomycosis is the most common nail disorder, accounting for up to 50% of all nail problems and about 30% of cutaneous fungal infections. Treatment of onychomycosis is expensive.
Sherin M. Emam, Osama H. Abd El-salam
doaj   +1 more source

Real-time PCR Primer sequences.

, 2022
Real-time PCR Primer sequences.
Richard Connor (143488), Tahseen Raza (10112696), Jason Liem (13794442), Jia Liu (41593), Erich A. Peterson (8716485), Martin Cannon (364845), Steven J. Conrad (2104483), Bernice Nounamo (13794445)   +7 more
core   +1 more source