Results 51 to 60 of about 19,571,554 (293)

Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCR

Kufa Journal for Veterinary Medical Sciences, 2016
Pseudomonas aeruginosa is an aerobic Gram-negative bacterium which has emerged as one of the most problematic nosocomial pathogens.To characterizes P.
Marrib N. Rasheed, Noor S. Latteef, Ashwak B. Jassim   +2 more
doaj   +1 more source

Desenvolvimento de iniciadores e sondas para detecção de cry1A.105 e cry2Ab2 e aplicação de PCR e PCR em tempo real para detecção de OGM em alimentos [PDF]

, 2011
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Ciência dos Alimentos, Florianópolis, 2011Reação em Cadeia da Polimerase (PCR) e PCR quantitativa em tempo real são usadas para detecção ...
Dinon, Andréia Zilio
core  

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon [PDF]

, 2008
PCR inhibition by nucleic acid extracts is a well known yet poorly described phenomenon. Inhibition assessment generally depends on the assumption that inhibitors affect all PCR reactions to the same extent; i.e.
Miller Robert F, Morris-Jones Stephen D, Tanya Novak, Garson Jeremy A, Jim F Huggett, Morris-Jones, SD, Zumla, A, Huggett, JF, Miller, RF, Green Clare, Zumla Alimuddin, Garson, JA, Novak Tanya, Novak, T, Jeremy A Garson, Clare Green, Stephen D Morris-Jones, Robert F Miller, Green, C, Huggett Jim F, Alimuddin Zumla   +20 more
core   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

A simple and cost-effective real-time PCR method using diluted and heat-treated whole blood lysate

Scientific Reports
Although DNA extraction is a crucial step before polymerase chain reaction (PCR), attempts to perform PCR without DNA isolation have been ongoing since the 1990s.
Gökçe Güllü Amuran, Büşra Polat, Abdülkadir Kahraman, Mustafa Akkiprik   +3 more
doaj   +1 more source

Development and verification of PCR based assay to dectect and quantify garden pea lec gene

Czech Journal of Food Sciences, 2012
Genetically modified organisms (GMOs) entering the food chain have became its part, which is necessary to monitor. GMO analyses are used as a control mechanism according to valid acquis communautaire for traceability and labeling of GMOs.
Aleš VRÁBLÍK, Jan HODEK, Josef SOUKUP, Kateřina DEMNEROVÁ, Jaroslava OVESNÁ   +4 more
doaj   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Evaluation of VERSANT® CT/GC DNA 1.0 Assay in conjunction with VERSANT® kPCR Molecular system

Microbiologia Medica, 2011
A total of 463 first void urine specimens were obtained from patients attending the Sexually Transmitted Diseases (STD) Outpatients Clinic of the St. Orsola University Hospital in Bologna, Italy. Samples were tested by the new test VERSANT® CT/GC DNA 1.0
Antonella Marangoni, Alessandra Moroni, Antonietta D’Antuono, Nicoletta Banzola, Enzo Della Bella, Claudio Foschi, Paola Nardini, Paola Monari, Manuela Donati, Roberto Cevenini   +9 more
doaj   +1 more source