Results 41 to 50 of about 20,355,262 (262)
Liver organoids: modelling complexity in homeostasis and disease
FEBS Letters, EarlyView.Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley +1 more source
A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control [PDF]
, 2005 We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of
Vázquez-Boland, JA +9 more
core
FEBS Letters, EarlyView.Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante +10 more
wiley +1 more source
Prevalence of bloodstream pathogens is higher in neonatal encephalopathy cases vs. controls using a novel panel of real-time PCR assays. [PDF]
, 2014 BACKGROUND: In neonatal encephalopathy (NE), infectious co-morbidity is difficult to diagnose accurately, but may increase the vulnerability of the developing brain to hypoxia-ischemia.
Klein Nigel +38 more
core +1 more source
Multicolor combinatorial probe coding for real-time PCR. [PDF]
PLoS ONE, 2011 The target volume of multiplex real-time PCR assays is limited by the number of fluorescent dyes available and the number of fluorescence acquisition channels present in the PCR instrument.
Qiuying Huang +8 more
doaj +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCR
Kufa Journal for Veterinary Medical Sciences, 2016 Pseudomonas aeruginosa is an aerobic Gram-negative bacterium which has emerged as one of the most problematic nosocomial pathogens.To characterizes P.
Marrib N. Rasheed +2 more
doaj +1 more source
FEBS Letters, EarlyView.Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil +3 more
wiley +1 more source
Real-time quantitative PCR primers.
, 2020 Real-time quantitative PCR primers.
Samantha A. Brooks (8693760) +4 more
core +1 more source
The application of PCR based methods in diagnostics of some viral infections of swine [PDF]
Biotechnology in Animal Husbandry, 2016 Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process.
Nišavić Jakov +4 more
doaj