Results 31 to 40 of about 19,571,554 (293)

Advanced and conventional molecular techniques in the diagnosis of Mycoplasma pneumoniae in patients with respiratory tract infection

مجلة كلية الطب, 2014
Background:-M. pneumoniae is an important human pathogen that produces community-acquired respiratory tract infection. Diagnosis of M. pneumoniae infection is challenging and crucial for the timely initiation of the effective antibiotic therapy ...
Muntaha M. Hassan, Mushtak T.S. Al-Ouqaili, Nidhal A. Mohammed, Omar A. Mohammed   +3 more
doaj   +1 more source

Internally controlled real-time PCR method for quantitative species-specific detection and vapA genotyping of Rhodococcus equi [PDF]

, 2006
We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen.
Makrai, L, D. A. Lewis, Rodríguez-Lázaro, AD, Scortti, MM, Rodríguez-Lázaro, David, Hernandez, Marta, Lewis, DA, Vazquez-Boland, Jose A, M. Hernandez, J. A. Vazquez-Boland, Makrai, Laszlo, U. Fogarty, Scortti, Mariela, Hernández, M, Navas, Jesus, Ocampo-Sosa, A, Vázquez-Boland, JA, J. Navas, Ocampo-Sosa, Alain A, Lewis, Deborah A, Fogarty, U, Fogarty, Ursula, M. Scortti, A. A. Ocampo-Sosa, L. Makrai, Navas, J, D. Rodriguez-Lazaro   +26 more
core   +1 more source

Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)

Revista Brasileira de Zootecnia, 2015
The objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens).
Shengdong Huo, Qijian Yuan, Taojie Zhang, Junlin Liu, Ayimuguli Abudureyimu, Jutian Yang   +5 more
doaj   +1 more source

Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1 [PDF]

Veterinarski Glasnik, 2010
Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard ...
Milić Nenad, Nišavić Jakov, Ašanin Ružica, Knežević Aleksandra, Ašanin Jelena, Vidanović D., Šekler Milanko   +6 more
doaj   +1 more source

Rapid and Sensitive Qualitative Duoplex Real-Time PCR Method for Discriminatory and Confirmatory Diagnosis of HTLV-1 and HTLV-2 Infections: Brazilian Multicentric Study

Frontiers in Medicine, 2022
Human T cell lymphotropic virus (HTLV) is the caustive agent of two main conditions i. e., the HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and the adult T-cell leukemia/lymphoma (ATLL).
Mauricio Cristiano Rocha-Junior, Mauricio Cristiano Rocha-Junior, Evandra Strazza Rodrigues, Evandra Strazza Rodrigues, Svetoslav Nanev Slavov, Svetoslav Nanev Slavov, Tatiane Assone, Maíra Pedreschi, Debora Glenda Lima de La Roque, Debora Glenda Lima de La Roque, Maisa Sousa, Viviana Olavarria, Bernardo Galvão-Castro, Benedito Antonio Lopes da Fonseca, Augusto César Penalva de Oliveira, Jerusa Smid, Oswaldo Massaiti Takayanagui, Jorge Casseb, Dimas Tadeu Covas, Dimas Tadeu Covas, Dimas Tadeu Covas, Simone Kashima, Simone Kashima, Simone Kashima   +23 more
doaj   +1 more source

A MIQE-Compliant Real-Time PCR Assay for Aspergillus Detection [PDF]

, 2012
PMCID: PMC3393739This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are ...
Bustin, SA, Johnson Gemma L., Bustin Stephen A., David F Bibby, Stephenie Wong, Wong, S, Agrawal Samir G., Agrawal, SG, Gemma L Johnson, Bibby, DF, Stephen A Bustin, Bibby David F., Samir G Agrawal, Johnson, GL, Wong Stephenie   +14 more
core   +2 more sources

Comparison of real-time PCR, conventional PCR and RT-lamp for the detection of Coconut Cadang-Cadang Viroid variant in oil palm

, 2023
Real-time quantitative polymerase chain reaction (real-time PCR) was designed for the detection of oil palm Coconut Cadang-cadang Viroid (CCCVd) 246 nt variant.
Roslan, Nur Diyana, Vadamalai, Ganesan, Idris, Abu Seman, Kong, Lih Ling, Sundram, Shamala   +4 more
core   +1 more source

Statistical analysis of real-time PCR data

BMC Bioinformatics, 2006
Background Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative real-time PCR are still lacking; specifically in the realm of appropriate statistical treatment.
Reed Ann, Yuan Joshua S, Chen Feng, Stewart C Neal   +3 more
doaj   +1 more source

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains [PDF]

Archives of Razi Institute, 2019
Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents.
S. Alamian, T. Zahraei Salehi, K. Aghaiypour Kolyani, M. Esmaelizad, A. Etemadi   +4 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source