Results 11 to 20 of about 1,770,911 (287)

Validation of the NAT Chagas IVD Kit for the Detection and Quantification of Trypanosoma cruzi in Blood Samples of Patients with Chagas Disease

Life, 2023
In the absence of validated biomarkers to control the cure of Chagas disease, PCR-based diagnosis is being used as the main tool for an early indication of therapeutic failure.
Otacilio C. Moreira, Alice Gomes Fernandes, Natalia Lins da Silva Gomes, Carolina Messias dos Santos, Thiago Jacomasso, Alexandre Dias Tavares Costa, Lucas de O. Rossetti Nascimento, Alejandro Marcel Hasslocher-Moreno, Pedro Emmanuel Alvarenga Americano do Brasil, Luis Gustavo Morello, Fabricio Klerynton Marchini, Marco Aurelio Krieger, Constança Britto   +12 more
doaj   +1 more source

RTPrimerDB : the portal for real-time PCR primers and probes [PDF]

, 2009
RTPrimerDB (http://www.rtprimerdb.org) is a freely accessible database and analysis tool for real-time quantitative PCR assays. RTPrimerDB includes records with user submitted assays that are linked to genome information from reference databases and ...
S. Lefever, J. Vandesompele, F. Speleman, F. Pattyn, Pattyn, Pattyn, Aranyi, Hellemans   +7 more
core   +2 more sources

MicroRNA and histopathological characterization of pure mucinous breast carcinoma

Cancer Biology & Medicine, 2013
Objective: To evaluate the short-term outcomes of video-assisted thoracic surgery (VATS) for thoracic tumors. Methods: The data of 1,790 consecutive patients were retrospectively reviewed.
Fang Li, Ning Li, Bai-Hua Zhang, Da-Wei Wang, Ke-Lin Sun, Gui-Yu Chen, Ju-Wei Mu, You-Sheng Mao et al.   +7 more
doaj   +1 more source

Enhanced analysis of real-time PCR data by using a variable efficiency model : FPK-PCR [PDF]

, 2011
Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples,
Goetghebeur, Els, Lievens, Antoon, Van Aelst, Stefan, Van den Bulcke, Marc   +3 more
core   +3 more sources

Prevalence and geographic distribution of Babesia conradae and detection of Babesia vogeli in free-ranging California coyotes (Canis latrans)

International Journal for Parasitology: Parasites and Wildlife, 2022
Babesia species are intraerythrocytic piroplasms that can result in disease characterized by hemolytic anemia and thrombocytopenia. Of the 5 species that are known to infect canids in the United States, Babesia conradae is most frequently diagnosed in ...
Nadia N. Javeed, Laura Shultz, Samantha Barnum, Janet E. Foley, Emir Hodzic, Emily L. Pascoe, Beatríz Martínez-López, Niamh Quinn, Danielle Bucklin, Jonathan D. Dear   +9 more
doaj   +1 more source

Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques [PDF]

, 2019
Background: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops.
Alfaro-Fernandez A., Caruso A. G., Davino S., Panno S., Ruiz-Ruiz S., San Ambrosio M. I. F.   +5 more
core   +1 more source

Handheld real-time PCR device

Lab on a Chip, 2016
World's smallest, fully autonomous, handheld real-time PCR was shown in this contribution. The device can quickly process up to four samples at a time with detection capability of a single DNA copy. The fully integrated system includes all required electronics for fluorescence measurement, data viewing (LCD display) and processing, and is ideal for use
Ahrberg, Christian D., Ilic, Bojan Robert, Manz, Andreas, Neužil, Pavel   +3 more
openaire   +4 more sources

Introducing automation to the molecular diagnosis of Trypanosoma cruzi infection: A comparative study of sample treatments, DNA extraction methods and real-time PCR assays [PDF]

, 2018
Background Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infection. The development of automated DNA extraction methodologies and PCR systems is an important step toward the standardization of protocols ...
Abras, Alba, Ballart, Cristina, Berenguer, Pere, Gascón, Joaquim, Gutiérrez, Cristina, Gállego, Montserrat, Llovet, Teresa, Muñoz, Carmen, Pinazo, María-Jesús, Posada, Elizabeth, Roig, Carme, Schijman, Alejandro Gabriel, Tebar, Silvia   +12 more
core   +3 more sources

REAL TIME PCR;

The Professional Medical Journal, 2012
In recent years, real-time PCR has come forward as a robust and widely used molecular technique in clinical and biologicalsettings. Although it can detect very minute quantities of target nucleic acid, but quantification of specific nucleic acids is not an easy task.Accurate and precise quantification is hampered by a number of factors that may include
ABIDA RAZA, NAUREEN A KHATTAK
openaire   +3 more sources

Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions [PDF]

, 2003
BACKGROUND: Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development of a ...
Bell, S.M., Bransfield, K., Combaret, V., Douglas, S.H., Field, S.L., Inglehearn, C.F., Isaacs, J.D., Leong, F.T., Markham, A.F., Mighell, A.J., Ponchel, F., Puisieux, A., Robinson, P.A., Toomes, C.   +13 more
core   +3 more sources