Results 11 to 20 of about 20,355,262 (262)

Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. [PDF]

open access: yes, 2008
peer-reviewedBackground: A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium, Kentucky and ...
Sheila McGuinness   +15 more
core   +1 more source

Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize [PDF]

open access: yes, 2012
Zearalenone (ZEA) is a mycotoxin produced by some species of Fusarium, especially by Fusarium graminearum and F. culmorum. ZEA induces hyperoestrogenic responses in mammals and can result in reproductive disorders in farm animals. In the present study, a
Atoui, Ali   +3 more
core   +1 more source

A Parylene Real Time PCR Microdevice [PDF]

open access: yes, 2010
The polymerase chain reaction (PCR) is a powerful biochemical assay that is used in virtually all biochemical labs. By specifically amplifying a small sample of DNA, this technique is useful in the fields of paternity testing, forensics, and virus ...
Quach, Quoc Chan (Brandon)
core   +1 more source

A real-time PCR assay to estimate Leishmania chagasi load in its natural sand fly vector Lutzomyia longipalpis. [PDF]

open access: yes, 2008
Leishmania chagasi, transmitted mainly by Lutzomyia longipalpis sand flies, causes visceral leishmaniasis and atypical cutaneous leishmaniasis in Latin America.
Ranasinghe, Shalindra   +9 more
core   +1 more source

Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms [PDF]

open access: yes, 2008
Background The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying genetically modified components in
Cankar, K.   +11 more
core   +1 more source

Evaluating an MRNA based body fluid identification test using sybr green fluorescent dye and real-time PCR [PDF]

open access: yes, 2011
The requirement to have more definitive and wider ranging body fluid identification (BFI) tests has resulted in a range of mRNA based real-time PCR BFI assays utilising Taqman fluorescent dye.
Connolly, Jo-Ann, Williams, Graham
core   +2 more sources

Real-time PCR for mRNA quantitation

open access: yesBioTechniques, 2005
Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to ...
Marisa L. Wong, Juan F. Medrano
doaj   +1 more source

Simulation of between repeat variability in real time PCR reactions. [PDF]

open access: yesPLoS ONE, 2012
While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process.
Antoon Lievens   +3 more
doaj   +1 more source

Development of real-time PCR for detection of Mycoplasma hominis [PDF]

open access: yes, 2004
Background: Mycoplasma hominis is associated with pelvic inflammatory disease, bacterial vaginosis, post partum fever, sepsis and infections of the central nervous system often leading to serious conditions.
Christiansen, G   +19 more
core   +1 more source

Real-time PCR Primer sequences.

open access: yes, 2022
Real-time PCR Primer sequences.
Richard Connor (143488)   +7 more
core   +1 more source

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