Results 111 to 120 of about 734,685 (383)

Unveiling unique protein and phosphorylation signatures in lung adenocarcinomas with and without ALK, EGFR, and KRAS genetic alterations

Molecular Oncology, EarlyView.
Proteomic and phosphoproteomic analyses were performed on lung adenocarcinoma (LUAD) tumors with EGFR, KRAS, or EML4–ALK alterations and wild‐type cases. Distinct protein expression and phosphorylation patterns were identified, especially in EGFR‐mutated tumors. Key altered pathways included vesicle transport and RNA splicing.
Fanni Bugyi, Mirjam Balbisi, Simon Sugár, Lóránd Váncza, Eszter Regős, Ilona Kovalszky, Ibolya Laczó, Tünde Harkó, Gábor Kecskeméti, Zoltán Szabó, Judit Moldvay, László Drahos, Lilla Turiák   +12 more
wiley   +1 more source

Breakthrough Solution for Antimicrobial Resistance Detection: Surface‐Enhanced Raman Spectroscopy‐based on Artificial Intelligence

Advanced Materials Interfaces, EarlyView., 2023
This review discusses the use of Surface‐Enhanced Raman Spectroscopy (SERS) combined with Artificial Intelligence (AI) for detecting antimicrobial resistance (AMR). Various SERS studies used with AI techniques, including machine learning and deep learning, are analyzed for their advantages and limitations.
Zakarya Al‐Shaebi, Munevver Akdeniz, Awel Olsido Ahmed, Mine Altunbek, Omer Aydin   +4 more
wiley   +1 more source

Validating Real-Time Polymerase Chain Reaction (PCR) Assays

, 2021
For many years, the development of assays took place in the laboratories where the test was required. Whilst more and more commercially developed tests have become available, novel assays continue to be developed in academic hospital laboratories. Although commercial kits are more expensive than LDTs, they enable the rapid introduction of assays, with ...
openaire   +2 more sources

Presence of infectious agents and co-infections in diarrheic dogs determined with a real-time polymerase chain reaction-based panel

BMC Veterinary Research, 2014
BackgroundInfectious diarrhea can be caused by bacteria, viruses, or protozoan organisms, or a combination of these. The identification of co-infections in dogs is important to determine the prognosis and to plan strategies for their treatment and ...
A. Gizzi, S. T. Oliveira, C. Leutenegger, M. Estrada, D. A. Kozemjakin, R. Stedile, M. Marcondes, A. Biondo   +7 more
semanticscholar   +1 more source

Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells

FEBS Open Bio, EarlyView.
Stem cell‐based embryo models (SCBEMs) are valuable to study early developmental milestones. Matrigel, a basement membrane matrix, is a critical substrate used in various SCBEM protocols, but its role in driving stem cell lineage commitment is not clearly defined.
Atoosa Amel, Rachel Brown, Alexa Rabeling, Mubeen Goolam   +3 more
wiley   +1 more source

Alcohol induces cell proliferation via hypermethylation of ADHFE1 in colorectal cancer cells [PDF]

, 2014
BACKGROUND: The hypermethylation of Alcohol dehydrogenase iron containing 1 (ADHFE1) was recently reported to be associated with colorectal cancer (CRC) differentiation.
Hye Jeong Lee, Hyeon Soo Kim, Ji Wook Moon, Jin Kim, Jung Ok Lee, Jung Seon Seo, Nami Kim, Soo Kyung Lee, Sun-Hwa Park, Yong Woo Lee   +9 more
core   +1 more source

Iron‐dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP‐1 monocytes

FEBS Open Bio, EarlyView.
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna, Richard Buchal, Martin Leníček, Amit Shachak, Jan Pláteník   +4 more
wiley   +1 more source

Real-time polymerase chain reaction for diagnosis and management of HIV-induced uveitis

Indian Journal of Ophthalmology, 2018
Intraocular (IO) inflammation in patients with Human immune deficiency virus (HIV) infection can be due to opportunistic infections, immune recovery uveitis, drugs used in the management or a primary manifestation of HIV itself. We studied the role of RT-
Jayapratha R Selvaraj, S Sudharshan, Lily K Therese, M K Janani, Poongulali Selvamuthu, Parveen Rewri, Jyotirmay Biswas   +6 more
doaj   +1 more source

Detection of clonal antigen receptor gene rearrangement in dogs with lymphoma by real-time polymerase chain reaction and melting curve analysis

BMC Veterinary Research, 2014
BackgroundMolecular techniques that detect canine lymphoma cells by their clonal antigen receptor gene rearrangement play an increasing role for diagnosis as well as for monitoring minimal residual disease during and after cytostatic therapy.
K. Langner, A. Joetzke, V. Nerschbach, N. Eberle, H. Schuberth, M. Koy, I. Nolte, D. Betz   +7 more
semanticscholar   +1 more source

Knockout of the mitoribosome rescue factors Ict1 or Mtrfr is viable in zebrafish but not mice: compensatory mechanisms underlying each factor's loss

FEBS Open Bio, EarlyView.
Mitochondria contain two mitoribosome rescue factors, ICT1 and MTRFR (C12orf65). ICT1 also functions as a mitoribosomal protein in mice and humans, and its loss is lethal. Although Mtrfr knockout mice could not be generated, knockout zebrafish lines for ict1 and mtrfr were established.
Nobukazu Nameki, Chika Tomisawa, Soichiro Hoshino, Hidehiko Shimizu, Masashi Abe, Sho Arai, Kanako Kuwasako, Naoki Asakawa, Yusuke Inoue, Takuro Horii, Izuho Hatada, Masakatsu Watanabe   +11 more
wiley   +1 more source